| 000 | 05945nam a22004815i 4500 | ||
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| 001 | 978-1-59259-853-3 | ||
| 003 | DE-He213 | ||
| 005 | 20260610083622.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 100301s2005 xxu| s |||| 0|eng d | ||
| 020 |
_a9781592598533 _9978-1-59259-853-3 |
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| 024 | 7 |
_a10.1385/1592598536 _2doi |
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| 050 | 4 | _aQD415-436 | |
| 072 | 7 |
_aPSB _2bicssc |
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_aSCI007000 _2bisacsh |
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_aPSB _2thema |
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| 082 | 0 | 4 |
_a572 _223 |
| 245 | 1 | 0 |
_aLaser Capture Microdissection _h[electronic resource] : _bMethods and Protocols / _cedited by Graeme I. Murray, Stephanie Curran. |
| 250 | _a1st ed. 2005. | ||
| 264 | 1 |
_aTotowa, NJ : _bHumana Press : _bImprint: Humana, _c2005. |
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| 300 |
_aXIV, 320 p. 82 illus., 1 illus. in color. _bonline resource. |
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| 336 |
_atext _btxt _2rdacontent |
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| 337 |
_acomputer _bc _2rdamedia |
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| 338 |
_aonline resource _bcr _2rdacarrier |
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| 347 |
_atext file _bPDF _2rda |
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| 490 | 1 |
_aMethods in Molecular Biology, _x1940-6029 ; _v293 |
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| 505 | 0 | _aAn Introduction to Laser-Based Tissue Microdissection Techniques -- Microdissection and DNA Analysis -- Methacarn Fixation for Genomic DNA Analysis in Microdissected Cells -- Multiplex Quantitative Real-Time PCR of Laser Microdissected Tissue -- comparative Genomic Hybridization Using DNA From Laser Capture Microdissected Tissue -- Detection of Ki-ras and p53 Mutations by Laser Capture Microdissection/PCR/SSCP -- Whole-Genome Allelotyping Using Laser Microdissected Tissue -- Microdissection for Detecting Genetic Aberrations in Early and Advanced Human Urinary Bladder Cancer -- Laser Microdissection for Microsatellite Analysis in Colon and Breast Cancer -- Assessment of RET/PTC Oncogene Activation in Thyroid Nodules Utilizing Laser Microdissection Followed by Nested RT-PCR -- Combined Laser-Assisted Microdissection and Short Tandem Repeat Analysis for Detection of In Situ Microchimerism After Solid Organ Transplantation Ulrich Lehmann, Anne Versmold, and Hans Kreipe -- RNA and Gene Expression Studies Using Microdissected Cells -- Laser-Assisted Microdissection of Membrane-Mounted Tissue Sections -- Laser-Assisted Microdissection of Membrane-Mounted Sections Following Immunohistochemistry and In Situ Hybridization -- Laser-Assisted Cell Microdissection Using the PALM System -- Laser Microdissection and RNA Analysis -- Gene Expression Profiling of Primary Tumor Cell Populations Using Laser Capture Microdissection, RNA Transcript Amplification, and GeneChip® Microarrays -- 16 Quantification of Gene Expression in Mouse and Human Renal Proximal Tubules -- Laser Capture Microdissection for Analysis of Macrophage Gene Expression From Atherosclerotic Lesions -- Analysis of Pituitary Cells by Laser Capture Microdissection -- Microdissection Techniques And Applications In Proteomics -- Laser CaptureMicrodissection and Colorectal Cancer Proteomics -- Proteomic Analysis of Human Bladder Tissue Using SELDI® Approach Following Microdissection Techniques -- Microdissection And Molecular Analysis Of Microorganisms -- Genetic Analysis of HIV by In Situ PCR-Directed Laser Capture Microscopy of Infected Cells -- Use of Laser Capture Microdissection Together With In Situ Hybridization and Real-Time PCR to Study Distribution of Latent Herpes Simplex Virus Genomes in Mouse Trigeminal Ganglia -- Laser Capture Microdissection and PCR for Analysis of Human Papilloma Virus Infection -- Laser Capture Microdissection of Hepatic Stages of the Human Parasite Plasmodium falciparum for Molecular Analysis. | |
| 520 | _aUse of laser microdissection has grown enormously to become a very powerful high-throughput technology essential to a range of downstream applications in pathology, cancer research, life sciences, medical diagnostics, and biotechnology. In Laser Capture Microdissection, expert laboratory researchers noted for their optimized methods describe in step-by-step detail not only how to successfully carry out tissue-based laser microdissection in their own laboratories using the different systems available, but also how to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. These readily reproducible methods make it possible to carry out contamination-free molecular analyses of specific types of normal and diseased cells. Highlights include gene expression profiling of primary tumor cell populations, RNA transcript amplification and GeneChip® microarrays, colorectal cancer proteomics, and proteomic analysis of human bladder tissue using the SELDI® approach. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Laser Capture Microdissection provides a highly practical guide to successful tissue-based laser microdissection using available commercial systems and to the interpretation of the results using a wide range of sophisticated molecular technologies. | ||
| 650 | 0 | _aBiochemistry. | |
| 650 | 0 | _aCytology. | |
| 650 | 1 | 4 | _aBiochemistry. |
| 650 | 2 | 4 | _aCell Biology. |
| 700 | 1 |
_aMurray, Graeme I. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 700 | 1 |
_aCurran, Stephanie. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 710 | 2 | _aSpringerLink (Online service) | |
| 773 | 0 | _tSpringer Nature eBook | |
| 776 | 0 | 8 |
_iPrinted edition: _z9781588292605 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781617374586 |
| 830 | 0 |
_aMethods in Molecular Biology, _x1940-6029 ; _v293 |
|
| 856 | 4 | 0 | _uhttps://doi.org/10.1385/1592598536 |
| 912 | _aZDB-2-PRO | ||
| 999 |
_c5091 _d5091 |
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