| 000 | 04740nam a22005055i 4500 | ||
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| 001 | 978-1-59259-565-5 | ||
| 003 | DE-He213 | ||
| 005 | 20260610083622.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 100301s1998 xxu| s |||| 0|eng d | ||
| 020 |
_a9781592595655 _9978-1-59259-565-5 |
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| 024 | 7 |
_a10.1385/0896034488 _2doi |
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| 050 | 4 | _aQD415-436 | |
| 072 | 7 |
_aPSB _2bicssc |
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| 072 | 7 |
_aSCI007000 _2bisacsh |
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| 072 | 7 |
_aPSB _2thema |
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| 082 | 0 | 4 |
_a572 _223 |
| 245 | 1 | 0 |
_aPolyamine Protocols _h[electronic resource] / _cedited by David M. L. Morgan. |
| 250 | _a1st ed. 1998. | ||
| 264 | 1 |
_aTotowa, NJ : _bHumana Press : _bImprint: Humana, _c1998. |
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| 300 |
_aIX, 186 p. _bonline resource. |
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| 336 |
_atext _btxt _2rdacontent |
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| 337 |
_acomputer _bc _2rdamedia |
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| 338 |
_aonline resource _bcr _2rdacarrier |
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| 347 |
_atext file _bPDF _2rda |
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| 490 | 1 |
_aMethods in Molecular Biology, _x1940-6029 ; _v79 |
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| 505 | 0 | _aPolyamines: An Introduction -- Assay Methods for Enzymes of Polyamine Biosynthesis -- Determination of Ornithine Decarboxylase Activity Using [3H]Omithine -- Assay of Mammalian Ornithine Decarboxylase Activity Using [14C]Ornithine -- Assay of Mammalian S-Adenosylmethionine Decarboxylase Activity -- Assay of Spermidine and Spermine Synthases -- Measurement of Spermidine/Spermine N 1-Acetyltransferase Activity -- Assay of Spermidine N 8-Acetyltransferase -- Assay Methods for Enzymes of Polyamine Catabolism -- Assay of N 8-Acetylspermidine Deacetylase -- Hydrogen Peroxide Assay for Amine Oxidase Activity -- Radiochemical Assay of Diamine Oxidase -- Radiochemical Estimation of Polyamine Oxidase -- Measurement of Aldehyde Formation as an Assay for Polyamine Oxidase Activity -- Measurement of Polyamines -- Determination of Polyamines as Their Benzoylated Derivatives by HPLC -- A Dansyl Chloride-HPLC Method for the Determination of Polyamines -- The Determination of Polyamines and Amino Acids by a Fluorescamine-HPLC Method -- Thin-Layer Chromatographic Method for Assaying Polyamines -- Measurement of Polyamine Transport -- Measurement of Polyamine Transport: Adherent Cells -- Measurement of Polyamine Transport: Cells in Suspension -- Measurement of Polyamine Efflux from Cells in Culture -- Measurement of Polyamine Effects on Cell Growth -- Leucine Incorporation and Thymidine Incorporation -- Tetrazolium (MTT) Assay for Cellular Viability and Activity. | |
| 520 | _aDavid Morgan brings together in Polyamine Protocols a unique collection of hands-on enzyme assay techniques for the study of polyamines and their biological functions. The techniques range from assay methods for enzymes of polyamine biosynthesis and catabolism to measurements of polyamines, polyamine transport, and polyamine effects on cell growth. All methods are presented by active researchers who have developed them to a high degree of perfection in their own laboratories, and include clear, step-by-step instructions with numerous hints and tips to ensure readily reproducible results. Polyamine Protocols is the only compilation of methods currently available for use in studies involving polyamines. The collection offers both novice and experienced researchers across many fields-including cell and molecular biology, pharmacology, oncology, physiology, laboratory medicine, and clinical pathology-all the tools needed to elucidate the biological roles played by these ubiquitous substances. | ||
| 532 | 8 | _aAccessibility summary: This PDF is not accessible. It is based on scanned pages and does not support features such as screen reader compatibility or described non-text content (images, graphs etc). However, it likely supports searchable and selectable text based on OCR (Optical Character Recognition). Users with accessibility needs may not be able to use this content effectively. Please contact us at accessibilitysupport@springernature.com if you require assistance or an alternative format. | |
| 532 | 8 | _aInaccessible, or known limited accessibility | |
| 532 | 8 | _aNo reading system accessibility options actively disabled | |
| 532 | 8 | _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com | |
| 650 | 0 | _aBiochemistry. | |
| 650 | 1 | 4 | _aBiochemistry. |
| 700 | 1 |
_aMorgan, David M. L. _eeditor. _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 710 | 2 | _aSpringerLink (Online service) | |
| 773 | 0 | _tSpringer Nature eBook | |
| 776 | 0 | 8 |
_iPrinted edition: _z9780896034488 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781617370427 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781489942739 |
| 830 | 0 |
_aMethods in Molecular Biology, _x1940-6029 ; _v79 |
|
| 856 | 4 | 0 | _uhttps://doi.org/10.1385/0896034488 |
| 912 | _aZDB-2-PRO | ||
| 999 |
_c5087 _d5087 |
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