000 04735nam a22005055i 4500
001 978-1-59259-579-2
003 DE-He213
005 20260610083622.0
007 cr nn 008mamaa
008 100301s1999 xxu| s |||| 0|eng d
020 _a9781592595792
_9978-1-59259-579-2
024 7 _a10.1385/0896035301
_2doi
050 4 _aRM300-666
072 7 _aMMG
_2bicssc
072 7 _aMED071000
_2bisacsh
072 7 _aMKG
_2thema
082 0 4 _a615
_223
245 1 0 _aReceptor Binding Techniques
_h[electronic resource] /
_cedited by Mary Keen.
250 _a1st ed. 1999.
264 1 _aTotowa, NJ :
_bHumana Press :
_bImprint: Humana,
_c1999.
300 _a304 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v106
505 0 _aOverview of Ligand-Receptor Binding Techniques -- Radioligand Binding in Different Preparations -- [3H]-GABA Binding to GABAA and GABAB Sites on Rat Brain Crude Synaptic Membranes -- Characterization of Imidazoline Receptors by Radioligand Binding -- Radioligand Binding to Solubilized 5-HT3 Receptors -- Solubilized Muscarinic Acetylcholine Receptors from the Rat Myocardium: Pharmacological and Hydrodynamic Characterization -- Radioligand Binding in Intact Cells -- Autoradiography of Peptide Receptors -- Measurement of Brain Gaba-Benzodiazepine Receptor Levels In Vivo Using Emission Tomography -- General Considerations -- Analysis of Binding Data -- Definition of Receptors in Radioligand-Binding Experiments -- The Use of Binding to Study Nonreceptor Sites -- Autoradiography of Enzymes, Second Messenger Systems, and Ion Channels -- Whole Human Brain Autoradiography of Serotonin Re-Uptake Inhibitors -- Measurement of Agonist-Stimulated [35S]GTP?S Binding to Cell Membranes -- mRNA: Detection by In Situ and Northern Hybridization.
520 _aIn Receptor Binding Techniques, detailed experimental protocols devised by expert practitioners demonstrate the power of radioligand binding for studying a wide variety of receptors. The methods can be applied to G protein-coupled receptors, receptors with integral ion channels, and sites other than receptors, such as guanine nucleotide binding proteins, enzymes, transporters, and mRNA. The individual protocols encompass many different preparations, ranging from solubilized receptors, through membrane preparations, intact cells, and autoradiography in tissue slices, to PET and SPECT analysis of binding in living human brain. Along with the detailed, easily reproducible instructions, the protocols also take into account the problems inherent in the basic binding technique, such as common experimental artifacts and the definition of receptor sites, as well as problems associated with the use of particular ligands and receptor preparations. Receptor Binding Techniques shows clearly how these techniques can be adapted to many different applications and fully utilized in drug discovery programs. It will be of high value not only to researchers new to the field of receptor binding, but also to more experienced workers, serving as an authoritative bench manual replete with optimalized practical methods and protocols. Pharmacologists, neuroscientists, cell biologists, molecular biologists, and biochemists.
532 8 _aAccessibility summary: This PDF is not accessible. It is based on scanned pages and does not support features such as screen reader compatibility or descriptions for non-text content (e.g., images and graphs). However, it likely supports searchable and selectable text based on OCR (Optical Character Recognition). Users with accessibility needs may not be able to use this content effectively. Please contact us at through this accessibility request webform if you require assistance or an alternative format.
532 8 _aInaccessible, or known limited accessibility
532 8 _aNo reading system accessibility options actively disabled
532 8 _aPublisher contact for further accessibility information: accessibilitysupport@springernature.com
650 0 _aPharmacology.
650 1 4 _aPharmacology.
700 1 _aKeen, Mary.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9780896035300
776 0 8 _iPrinted edition:
_z9781489942401
776 0 8 _iPrinted edition:
_z9781489942418
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v106
856 4 0 _uhttps://doi.org/10.1385/0896035301
912 _aZDB-2-PRO
999 _c5076
_d5076