TY  - BOOK
AU  - Kohler,Jennifer J.
AU  - Patrie,Steven M.
ED  - SpringerLink (Online service)
TI  - Mass Spectrometry of Glycoproteins: Methods and Protocols 
T2  - Methods in Molecular Biology,
SN  - 9781627031462
AV  - QD431-431.7 
U1  - 572.6 23
PY  - 2013///
CY  - Totowa, NJ
PB  - Humana Press, Imprint: Humana
KW  - Proteins 
KW  - Mass spectroscopy
KW  - Protein Biochemistry
KW  - Mass Spectrometry
N2  -  Glycosylation is the most abundant post-translational modification of proteins.  Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics.  These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol  is  an essential resource for those who work at the interface of glycobiology and mass spectrometry
UR  - https://doi.org/10.1007/978-1-62703-146-2
ER  -