TY  - BOOK
AU  - Michels,Paul A.M.
AU  - Ginger,Michael L.
AU  - Karnkowska,Anna
AU  - McCall,Laura-Isobel
AU  - Silber,Ariel M.
ED  - SpringerLink (Online service)
TI  - Euglenozoa: Methods and Protocols, Volume 1 
T2  - Methods in Molecular Biology,
SN  - 9781071651421
AV  - QR65-69 
U1  - 579.028 23
PY  - 2026///
CY  - New York, NY
PB  - Springer US, Imprint: Humana
KW  - Microbiology
KW  - Technique
KW  - Parasitology
KW  - Microbiology Techniques
N1  - Why Euglenozoans -- Isolation and Cultivation of Diplonemids -- Preparation of Diplonemid Samples for Microscopy -- Protocol for the Molecular Identification of Autotrophic Euglenids in Environmental Samples -- Culture and Genetic Modification of the Monoxenous Trypanosomatids Crithidia fasciculata and Crithidia bombi -- Poly(A) Profiling for Euglenozoans Using Oxford Nanopore Direct RNA Sequencing, Illustrated by the Example of Euglena gracilis -- Euglenid Extrachromosomal DNA: Assembly and Annotation -- Nuclear Genome Assembly and Annotation of Kinetoplastids -- Assembly and Annotation of Kinetoplastid and Diplonemid Mitochondrial Genomes -- Cleavage Under Targets and Release Using Nuclease (CUT&RUN) for Trypanosoma brucei Parasites -- Mapping Open Chromatin in Trypanosoma brucei Using ATAC-Seq -- Transcriptomic Analysis of Trypanosomatids -- Small RNA Profiling in Trypanosomes -- Desorption Electrospray Ionization–Mass Spectrometry Imaging (DESI-MSI) of Metabolites in Heart Samples Infected with Trypanosoma cruzi -- Microbiome Profiling in Chagas Disease: Sample Collection, Sequencing, and Analysis -- Metabolomics Analysis of Trypanosomes Using Ion Mobility-Enhanced Mass Spectrometry -- Exploring Protein Interactomes Using TurboID-Directed Proximity Labeling and Mass Spectrometry -- Mapping Trypanosoma Protein Interactome by Proximity-Dependent Biotinylation -- From Genome to Function: Metabolism-Wide Models of Lifecycle Stages of Parasites -- Enhanced Detection of Homology Using Artificial Intelligence in Euglenids
N2  - This detailed collection covers methods recently developed for experiments on organisms from each of the major euglenozoan groups, including diplonemids, euglenids, and kinetoplastids. Volume 1 presents a historical review of the research into euglenozoans, followed by sections of techniques involving isolation and culturing of different species of euglenozoans, as well as a variety of -omics research methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Euglenozoa: Methods and Protocols, Volume 1 serves as an ideal guide for researchers working with these fascinating organisms and investigating the aspects of their extreme biology. Chapter 14 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com
UR  - https://doi.org/10.1007/978-1-0716-5142-1
ER  -