TY  - BOOK
AU  - Cupples Connon,Catherine
ED  - SpringerLink (Online service)
TI  - Forensic DNA Analysis: Methods and Protocols 
T2  - Methods in Molecular Biology,
SN  - 9781071632956
AV  - QH426-470 
U1  - 576.5 23
PY  - 2023///
CY  - New York, NY
PB  - Springer US, Imprint: Humana
KW  - Genetics
KW  - Medical jurisprudence
KW  - Genetics and Genomics
KW  - Forensic Medicine
N1  - Forensic DNA Analysis: An Overview of the Laboratory Process -- Organic Extraction of Nucleic Acids using Ethanol Precipitation or Microcon® Centrifugal Filter Purification Methods -- Manual Silica-Based DNA Extractions -- Applied Biosystems™ PrepFiler™ Forensic DNA Extraction Kit (Manual and Semi-Automated via AutoMate Express™) -- Robotic DNA Extraction Utilizing Qiagen BioSprint® 96 Workstation -- DNA Extraction of Bone through Demineralization -- Differential Extraction with Purification via Organic/Microcon® and Promega DNA IQ™ Methods -- DNA Purification from Bloodstains and Buccal Cells/Saliva on FTA® Cards -- Yield Gel via Quantitative Gel Electrophoresis -- Quantitative PCR of Alu Repeats using PowerUp™ SYBR® Green Master Mix -- Quantitation of DNA using the Applied Biosystems Quantifiler® Trio DNA Quantification Kit -- QIAGEN's Investigator® Quantiplex Pro Kit -- DNA Amplification using Promega's PowerPlex® Fusion Systems (5C and 6C) -- Amplification of Extracted DNA and Direct Amplification with the PowerPlex® Y23 System -- Applied Biosystems' GlobalFiler™ PCR Amplification Kit -- QIAGEN’s Investigator® 24plex QS and GO! PCR Amplification -- Low Volume STR Amplification Options: Coupling with Standard or Fast PCR, Traditional or Normalized DNA Extraction, and/or Traditional or Alternative Capillary Electrophoresis -- Capillary Electrophoresis with Applied Biosystems' 3500 Genetic Analyzer -- Likelihood Ratio Calculation using LRmix Studio -- Mitochondrial DNA Analysis -- An Optimized Forensic DNA Analysis Workflow for Obtaining STR Results from Archived Latent Fingerprints -- Detection of Latent DNA using a DNA Binding Dye -- Rapid DNA Profile Development with Applied Biosystems RapidHIT™ ID System -- Next-Generation Sequencing: ForenSeq™ DNA Signature Prep Kit with the Illumina MiSeq FGx
N2  - This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures using both home-brew and commercial products. The chapters also discuss probabilistic modeling software and specialized start-to-finish procedures for mitochondrial DNA analysis, archived latent fingerprints, latent DNA, rapid DNA profiling, and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchersinterested in learning more about forensic DNA analysis procedures
UR  - https://doi.org/10.1007/978-1-0716-3295-6
ER  -