TY  - BOOK
AU  - Friedrich,Oliver
AU  - Gilbert,Daniel F.
ED  - SpringerLink (Online service)
TI  - Cell Viability Assays: Methods and Protocols 
T2  - Methods in Molecular Biology,
SN  - 9781071630525
AV  - QH573-671 
U1  - 571.6 23
PY  - 2023///
CY  - New York, NY
PB  - Springer US, Imprint: Humana
KW  - Cytology
KW  - Biological assay
KW  - Cell Biology
KW  - Assay Systems
N1  - Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness -- The MTT Assay: A Method for Error Minimization and Interpretation in Measuring Cytotoxicity and Estimating Cell Viability -- Assaying Proliferation Characteristics of Cells Cultured Under Static Versus Periodic Conditions -- Network Reconstruction as a Novel High-Level Marker of Functional Neuronal Viability -- Assaying Mitochondrial Function by Multi-Parametric Flow Cytometry -- High-Efficiency Single Cell Electrical Impedance Spectroscopy -- Cell Viability Multiplexing: Quantification of Cellular Viability by Barcode Flow Cytometry and Computational Analysis -- Average Rheological Quantities of Cells in Monolayers -- Assaying Spontaneous Network Activity and Cellular Viability Using Multi-Well Microelectrode Arrays -- Quantitative Live-Cell Ca2+ Imaging During Isotropic Cell Stretch -- Assessment of Cell Viability in Electrically Excitable Muscle Cells through Intact Twitch Stimulation.-Evaluating Cellular Viability by iTRAQ Proteomic Profiling -- Quantifying pH in Malaria Using pHluorin and Flow Cytometry -- Cell Membrane State, Permeability, and Elasticity Assessment for Single Cells and Cell Ensembles -- Neutral Red Uptake Assay to Assess Cytotoxicity In Vitro -- Digital Holographic Microscopy to Assess Cell Behavior -- Second Harmonic Generation Morphometry of Muscle Cytoarchitecture in Living Cells -- Optimization of Cell Viability Assays for Drug Sensitivity Screens -- Cellasys #8: A Microphysiometric Assay for Real-Time Cell Analysis within 24 Hours -- Live Cell Adhesion, Migration, and Invasion Assays -- Cell Viability and Immunogenic Function of T Cells Loaded with Nanoparticles for Spatial Guidance in Magnetic Fields -- Transwell In Vitro Cell Migration and Invasion Assays -- Live Cell Calcium Imaging of Non-Adherent Cells -- FUCCI Reporter Gene-Based Cell Cycle Analysis -- Cell Viability Assays for 3D Cellular Constructs -- Toxicological Analysis by Assessment of Vascularization and Cell Viability Using the Chicken's Chorioallantoic Membrane (CAM Assay) -- Assessment of Tissue Viability by Functional Imaging of Membrane Potential -- Multiplexed Viability Assays for High-Throughput Screening of Spheroids of Multiple Sizes -- Tracking Gut Motility in Organ and Cultures -- Optimized Method of 3D Scaffold Seeding, Cell Cultivation, and Monitoring Cell Status for Bone Tissue Engineering
N2  - This updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development with topics and assays that target cell viability, mobility, and functionality of tissues and organs, natural or bioartificial, in 3D. The book’s contents span a wide range of viability and functionality assays, from impedance spectroscopy to chemiluminescence, fluorescence and label-free optical detection methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Cell Viability Assays: Methods and Protocols, Second Edition serves as a valuable resource to the growing community in bioinspired life sciences, biomedical sciences, and biotechnology by providing more standardized protocols to probe the “wellbeing” of cells in various environments
UR  - https://doi.org/10.1007/978-1-0716-3052-5
ER  -