Structured Illumination Microscopy -- Multicolor super-resolution fluorescence imaging of cells -- Super-resolution spinning-disk confocal microscopy using optical photon reassignment -- Improving STED-based super-resolution-imaging with fluorescence lifetimes -- Single Molecule Localization Microscopy with Fixed Photoactivatable Fluorescent Proteins and Direct Stochastic Optical Reconstruction Microscopy -- Single particle tracking Photo-Activated Localization Microscopy -- Single Molecule Localisation Microscopy in the nucleus with self-labelling tags: Halo, SNAP and CLIP tags -- Single Molecule Localisation Microscopy in vivo at the Drosophila neuromuscular junction -- Tracking synaptic vesicles in live neurons using single-molecule super-resolution microscopy -- Single molecule imaging of endogenous proteins -- Shadow imaging of brain microanatomy in live and fixed tissue -- NanoPlex: A universal strategy to multiplex immunostainings for fluorescence microscopy using secondary nanobodies -- Spatiotemporal analysis of super-resolved live cell molecular trajectory data using nanoscale spatiotemporal indexing clustering -- Case study: Illuminating the nanoscale world of microbiology.

This volume provides comprehensive methods used to perform super-resolution microscopy and its analysis. Each chapter is written by a leading expert in the field, covering fundamentals of super-resolution microscopy, single-molecule localization microscopy (SMLM), stimulated emission depletion (STED) microscopy, expansion microscopy, and others. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Super-Resolution Microscopy: Methods and Protocols provides well-established protocols used by both academics and industrials.

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