Isolation and Maintenance in Culture of Primary Human Trophoblast from term Placentae -- Isolation of Human Villous Cytotrophoblastic Cells from Term Placenta for Transfection -- Enzymatic Digestion and Single Cell Isolation of Peri-Implantation Stage Human Trophoblast Cells -- Precision-Cut Slice Culture Method for Rat Placenta -- Evaluation of Molecular Interactions and Cellular Dynamics at the Maternal-Fetal Interface during Placental Morphogenesis -- In Vivo Quantitative Assessment of Gestational Choriocarcinoma Development and Progression using Luminescent Trophoblast Cells -- Analyzing Trophoblast Fusion using Immunofluorescence and Split Protein Complementation Assays -- Quantification of Trophoblast Syncytialization by Fluorescent Membrane Labeling -- Assessment of Placental Sodium Independent Leucine Uptake and Transfer in Trophoblast Cells -- Assessing Cholesterol Efflux on Primary Human Trophoblast Cells -- RGD-Based Fluorescenceto Assess Placental Angiogenesis -- Real-Time Assessment of Mitochondrial Function in Cytotrophoblast and Syncytialized Trophoblast Cells using the Seahorse XFe24 Extracellular Flux Analyzer -- Quantifying Trophoblast Function using the xCELLigence System -- Liposome Encapsulated Anti-Inflammatory Proteins for targeted Delivery to the Placenta to Treat Fetal Growth Restriction -- Trophoblast-Targeted Lipsomes for Placenta-Specific Drug Delivery -- Methods for Co-Culture of Primary Human Extravillous Trophoblast Cells and Uterine Natural Killer Cells -- Trophoblast Organoids as a Novel Tool to Study Human Placental Development and Function -- A Three-Dimensional Trophoblast Invasion Microfluidic Platform for Toxicological Screening -- Three-Dimensional In Vitro Human Placental Organoids from Mononuclear Villous Trophoblasts or Trophoblast Stem Cells to Understand Trophoblast Dysfunction in Fetal Growth Restriction.

This volume explores the latest approaches used to assess trophoblast angiogenesis, transport function, cellular respirations, migration, and invasion. The chapters in this book cover topics such as various methods to study and manipulate primary trophoblast cells; strategies using liposomes to deliver biomolecules to trophoblasts; in vitro models of the placenta that emulate the cellular interactions in the 3D uterine environment; and models exploring heterogenous cell types and 3D-organoid structures that represent cell-to-cell interactions in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Trophoblasts: Methods and Protocols is a valuable resource that will help researchersbetter understand the function of the placenta and its complex contribution to maternal health and fetal development, and also provide greater insights to the area of study known as the developmental origins of health and diseases (DoHaD). .

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