Simultaneous Determination of ABA, IAA, SA, and JA by UPLC-ESI-MS/MS -- Simultaneous Quantification of Salicylic Acid, Pipecolic Acid, and N-Hydroxypipecolic Acid in Plants Using High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry -- Comprehensive Evaluation of Abscisic Acid Content in Plants Through Quantitative and Qualitative Techniques -- Characterization and Quantification of Strigolactones in Root Exudates -- LC-MS Analysis and Quantification of Plants Apocarotenoids -- Extraction and Analysis of Cytokinins from Mammalian Tissues by HPLC–HRMS -- In Vivo Detection of IAA Using IAA Nanosensor -- Gene Expression Analysis to Investigate the Response of Salicylic Acid in Plant Immunity -- Practical RNA-Seq with Spike-Ins: A Bench-to-Bioinformatics Guide -- Single-Cell/Nucleus RNA-Sequencing for Phytohormone Signaling in Plants -- Single-Cell Transcriptomics in Arabidopsis in Response to Salicylic Acid and Jasmonic Acid -- Stereo-Seq Transcriptomics in Arabidopsis Leaves in Response to Salicylic Acid -- Automated Flow-Cytometric Readout of Reporter-Gene Activation in Transiently Transformed Protoplasts -- Protocols for Chromatin Immunoprecipitation Sequencing and qPCR to Investigate Pytohormone-Responsive Transcriptional Regulation in Plants -- Detection of tRNA-Derived Small RNAs in Arabidopsis thaliana via Northern Blotting: Exploring Potential Biomarkers for Phytohormone Analysis -- Deciphering Protein Interaction Dynamics Using the Yeast Three-Hybrid System -- Simplified In Vitro Assay for Testing Chemicals with Auxin Activity Using Direct Protein-Protein Interaction -- Root Growth and Leaf Senescence Assays as Physiological Readouts of Cytokinin Response in Arabidopsis thaliana.

This detailed collection compiles established and cutting-edge experimental methods and protocols in plant hormone research, indispensable for understanding the role of hormones in governing various aspects of plant growth, development, reproduction, and responses to environmental cues. The book offers insights into recent discoveries in phytohormones, ranging from the initial isolation and quantification techniques to cutting-edge molecular methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Hormones: Methods and Protocols serves as an ideal guide to furthering research into this cornerstone of modern plant science.

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