How to Approach the Isolation of a Natural Product -- Initial Extraction and Product Capture -- Supercritical Fluid Methods -- Isolation by Low-Pressure Column Chromatography -- Isolation by Ion-Exchange Methods -- Isolation by Preparative HPLC -- Isolation by Planar Chromatography -- Separation by High-Speed Countercurrent Chromatography -- Crystallization and Final Stages of Purification -- Dereplication and Partial Identification of Natural Products -- Purification of Water-Soluble Natural Products -- Special Problems with the Extraction of Plants -- Isolation of Marine Natural Products -- Scale-Up of Natural Products Isolation -- Follow-Up of Natural Product Isolation.

Natural Products Isolation provides a comprehensive introduction to techniques for the extraction and purification of natural products from all biological sources. The book opens with an introduction to separations and chromatography and discusses the approach to an isolation. Experienced experimentalists describe a wide array of methods for isolation of both known and unknown natural products, including initial extraction, open column chromatography, HPLC, countercurrent and planar chromatography, SFE, and crystallisation. Later chapters address specific issues in working with plants, marine organisms, water-soluble compounds, as well as scale-up, dereplication and follow-up of a natural product lead. For the less experienced, these chapters provide background information and hands-on advice - using real examples - about how to approach an extraction in general, when and how to apply a particular technique and how to modify conditions according to the nature of the compound being isolated. Geared to scientists with little experience of natural products extraction, but offering even skilled researchers valuable advice and insight, Natural Products Isolation lays the foundation for the potential extractor to isolate natural substances efficiently. Its methods and guidance will almost certainly play a major role in today's natural product discovery and development.

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