Activity-Based Protein Profiling – From Chemical Novelty to Biomedical Stalwart -- A Quantitative Mass-Spectrometry Platform to Monitor Changes in Cysteine Reactivity -- Activity-Based Protein Profiling with Natural Product-Derived Chemical Probes in Human Cell Lysates -- Inhibitor Discovery by Convolution Activity-Based Protein Profiling -- Activity-Based Protein Profiling in Bacteria -- Activity-Based Lipid Esterase Profiling of M. Bovis BCG at Different Metabolic States Using Tetrahydrolipstatin (THL) as Bait -- Comparative Activity-Based Flavin-Dependent Oxidase Profiling -- Activity-Dependent Photoaffinity Labelling of Metalloproteases -- Profiling the Activity of Deubiquitinating Enzymes using Chemically Synthesized Ubiquitin-Based Probes -- Profiling Protein Sumoylation using Biochemical and Mass Spectrometry-Based Research Tools -- Live Cell Imaging and Profiling of Cysteine Cathepsin Activity using a Quenched Activity-Based Probe -- Competitive Activity-Based Protein Profiling Of Serine Hydrolases: A Case Study on DAGL-Alpha -- A Protocol for Protein Profiling using Chemoselective Cleavable Linker Probes in Semi-Permeabilized Cells -- Cleavable Linkers in Chemical Proteomics Applications -- Two-Step Activity-Based Protein Profiling With the Proteasome System as Model of Study.

This volume focuses on explorative activity-based proteomics, biomedical applications of activity-based proteomics, and chemical strategies in activity-based proteomics providing a concise overview of activity-based protein profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Activity-Based Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.