Forensic DNA Analysis: An Overview of the Laboratory Process -- Organic Extraction of Nucleic Acids using Ethanol Precipitation or Microcon® Centrifugal Filter Purification Methods -- Manual Silica-Based DNA Extractions -- Applied Biosystems™ PrepFiler™ Forensic DNA Extraction Kit (Manual and Semi-Automated via AutoMate Express™) -- Robotic DNA Extraction Utilizing Qiagen BioSprint® 96 Workstation -- DNA Extraction of Bone through Demineralization -- Differential Extraction with Purification via Organic/Microcon® and Promega DNA IQ™ Methods -- DNA Purification from Bloodstains and Buccal Cells/Saliva on FTA® Cards -- Yield Gel via Quantitative Gel Electrophoresis -- Quantitative PCR of Alu Repeats using PowerUp™ SYBR® Green Master Mix -- Quantitation of DNA using the Applied Biosystems Quantifiler® Trio DNA Quantification Kit -- QIAGEN's Investigator® Quantiplex Pro Kit -- DNA Amplification using Promega's PowerPlex® Fusion Systems (5C and 6C) -- Amplification of Extracted DNA and Direct Amplification with the PowerPlex® Y23 System -- Applied Biosystems' GlobalFiler™ PCR Amplification Kit -- QIAGEN’s Investigator® 24plex QS and GO! PCR Amplification -- Low Volume STR Amplification Options: Coupling with Standard or Fast PCR, Traditional or Normalized DNA Extraction, and/or Traditional or Alternative Capillary Electrophoresis -- Capillary Electrophoresis with Applied Biosystems' 3500 Genetic Analyzer -- Likelihood Ratio Calculation using LRmix Studio -- Mitochondrial DNA Analysis -- An Optimized Forensic DNA Analysis Workflow for Obtaining STR Results from Archived Latent Fingerprints -- Detection of Latent DNA using a DNA Binding Dye -- Rapid DNA Profile Development with Applied Biosystems RapidHIT™ ID System -- Next-Generation Sequencing: ForenSeq™ DNA Signature Prep Kit with the Illumina MiSeq FGx.

This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures using both home-brew and commercial products. The chapters also discuss probabilistic modeling software and specialized start-to-finish procedures for mitochondrial DNA analysis, archived latent fingerprints, latent DNA, rapid DNA profiling, and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchersinterested in learning more about forensic DNA analysis procedures.

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