Protocol for the Systematic Fixation, Circuit-Based Sampling, and Qualitative and Quantitative Neuropathological Analysis of Human Brain Tissue -- Extraction and Purification of Single Nuclei from Frozen Human Brain Tissue -- Isolation of Human Microglia from Neuropathologically Diagnosed Cases in the Single-Cell Era -- Microglia in Human Postmortem Brain Samples: Quantitative Ultrastructural Analysis of Scanning Electron Microscopy Images -- Three-Dimensional Imaging of Fibrinogen and Neurovascular Alterations in Alzheimer’s Disease -- Human Induced Pluripotent Stem Cell (hiPSC)-Derived Neurons and Glia for the Elucidation of Pathogenic Mechanisms in Alzheimer’s Disease -- Modeling Alzheimer’s Disease using Human Brain Organoids -- 3D Biohybrid StarPEG-Heparin Hydrogel Cultures for Modeling Human Neuronal Development and Alzheimer’s Disease Pathology -- Computational Approaches to Assess Abnormal Metabolism in Alzheimer’s Disease Using Transcriptomics -- FISHing for Chromosome Instability and Aneuploidy in the Alzheimer’s Disease Brain -- Somatic CNV Detection by Single-Cell Whole-Genome Sequencing in Post-Mortem Human Brain -- Mass Spectrometry Analysis of the Human Brain Sphingolipidome -- Mass Spectrometry for the Advancement of Lipid Analysis in Alzheimer’s Research -- The Use of Affinity Purification-Mass Spectrometry to identify Phosphorylates Tau Interactors in Alzheimer’s Disease -- In Vitro Amplification of Pathogenic Tau Seeds from Neurodegenerative Disease Patient Brains -- Aβ and Tau Prions Causing Alzheimer’s Disease.

This volume explores the latest techniques used to study the human brain towards understanding Alzheimer’s Disease and related neurodegenerative disorders. Contributed to by world-renowned experts, the chapters in this book are divided into five parts. Part One discusses human post-mortem brain preparations including single-cell isolation and use of specialized imaging. Part Two talks about neural cellular models using primary and human induced pluripotent stem cells to model aspects of the human brain. Part Three details nucleic acid analyses including transcriptomic and somatic genomic changes, and Part Four discusses lipid analyses via mass spectrometry. Lastly, Part Five covers protein analyses, particularly Aβ and Tau. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tipson troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Alzheimer’s Disease: Methods and Protocols is a valuable tool for all researchers who want to expand their knowledge and understanding of this disease and other related dementias.

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