In Vitro Blood-Brain Barrier Functional Assays in a Human iPSC-Based Model -- In Vitro Techniques for Assessing Neurotoxicity using Human iPSC-Derived Neuronal 1 Models -- Oxidative Stress Signatures in Human Stem Cell Derived Neurons -- Glial Reactivity in Response to Neurotoxins: Relevance and Methods -- Neuron-Glia Interaction Studied with In Vitro Co-Culture -- Assessment of Mitochondrial Stress in Neurons: Proximity Ligation Assays to Detect Recruitment of Stress-Responsive Proteins to Mitochondria -- Epigenetic Changes in Cultured Neurons and Astrocytes -- The Neurosphere Assay as an In Vitro Method for Developmental Neurotoxicity (DNT) Evaluation -- Zebrafish as a Tool to Assess Developmental Neurotoxicity -- Rat Brain Slices: An Optimum Biological Preparation for acute Neurotoxicological Studies -- Electrophysiological Neuromethodologies -- A Method for Sampling Rat Cerebrospinal Fluid with Minimal Blood Contamination: A CriticalTool for Biomarker Studies -- Transporter Studies: Brain Punching Technique -- MicroRNA as a Marker for In Vitro Neurotoxicity Testing and Related Neurological Disorders -- Application of Non-Animal Methods to More Effective Neurotoxicity Testing for Regulatory Purposes.

This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field.