Systems Microbiology and Biomanufacturing (SMAB) aims at providing a platform for high-quality research in the interdisciplinary areas of industrial microbiology and biomanufacturing process development. Papers of SMAB deal with the following aspects: Industrial Microbiology and Biomanufacturing Microbial cell factories, evolutionary engineering, intelligent biocontrol technology, bioprocess engineering and bioreactor, and biosensor. Microbiome and Systems Metabolic Engineering Rational and systems metabolic engineering, lactic acid bacteria and probiotics, gene assembly and genome engineering, and microbiome for fermentation. Artificial Intelligence and Synthetic Biology Machine learning and computation-aided design of biomolecules, synthetic gene expression regulatory elements, genetic pathway regulation, and natural-product biosynthesis. Protein Engineering and Biocatalysis Recombinant protein expression, protein engineering, enzymology, directed evolution, and biocatalysis. Biorefineries and Waste Biotechnology Bioconversion of one-carbon compounds, biofuels production, algal biofuels and biorefinery, biogas, biohydrogen, biomass and feedstocks utilization, and waste treatment and management.