Medical BioMethods Handbook (Record no. 8534)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04663nam a22004575i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-59259-870-0 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083722.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 100301s2005 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781592598700 |
| -- | 978-1-59259-870-0 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1385/1592598706 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QH573-671 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSF |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI017000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSF |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 571.6 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Medical BioMethods Handbook |
| Medium | [electronic resource] / |
| Statement of responsibility, etc. | edited by John M. Walker, Ralph Rapley. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 2005. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Totowa, NJ : |
| Name of producer, publisher, distributor, manufacturer | Humana Press : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2005. |
| 300 ## - online resource | |
| Extent | XVI, 644 p. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Springer Protocols Handbooks, |
| International Standard Serial Number | 1949-2456 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Basic Techniques in Molecular Biology -- Probe Design, Production, and Applications -- Restriction Enzymes -- Southern Blotting as a Diagnostic Method -- Western Blotting as a Diagnostic Method -- Principles and Medical Applications of the Polymerase Chain Reaction -- Single-Strand Conformation Polymorphism (SSCP) Analysis -- Denaturing Gradient Gel Electrophoresis (DGGE) -- Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing -- to Capillary Electrophoresis of DNA -- Mapping Techniques -- Dideoxyfingerprinting for Mutation Detection -- Conformation-Sensitive Gel Electrophoresis -- Amplification Refractory Mutation System and Molecular Diagnostics -- Ligase Chain Reaction -- Chemical Cleavage of Mismatch -- The Protein Truncation Test -- Linkage, Allele Sharing, and Association -- Single-Nucleotide Polymorphisms -- cDNA Microarrays -- Nucleic Acid Sequence-Based Amplification -- Oligonucleotide Ligation Assay -- Quantitative TaqMan Real-Time PCR -- Use of Denaturing High-Performance Liquid Chromatography in Molecular Medicine -- Quantitative PCR -- Liquid Chromatography-Mass Spectrometry of Nucleic Acids -- Comparative Genomic Hybridization in Clinical and Medical Research -- Bioinformatic Tools for Gene and Protein Sequence Analysis -- In SituHybridization -- Enzyme-Linked Immunosorbent Assay -- Protein Therapeutics -- Karyotyping -- Microsatellite Analysis -- Analysis of Chromosomal Translocations -- Differential Display -- Techniques for Gene Expression Profiling -- Capillary Electrophoresis in Clinical Analysis -- Flow Cytometry in the Biomedical Arena -- Immunocytochemistry -- Ribotyping in Clinical Microbiology -- Diagnostic Applications of Protein Microarrays -- The Human Genome Project -- Prenatal Diagnosis of Inborn Errors of Metabolism -- Gene Therapy. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Powerful new techniques in molecular biology have revolutionized our ability to investigate and define cellular processes at the molecular level for medical purposes. In Medical Biomethods Handbook, John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are Southern and Western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy. State-of-the-art and highly practical, Medical Biomethods Handbook offers novice and experienced researchers an in-depth understanding of the theory, practice, and application of the key molecular biology techniques used in biomedical research and diagnostic investigation today. . |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Cytology. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Cell Biology. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Walker, John M. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rapley, Ralph. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781588292889 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781588293343 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Springer Protocols Handbooks, |
| International Standard Serial Number | 1949-2456 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1385/1592598706">https://doi.org/10.1385/1592598706</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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