Human Retrovirus Protocols (Record no. 8522)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 06558nam a22004455i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-59259-907-3 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083722.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 100301s2005 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781592599073 |
| -- | 978-1-59259-907-3 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1385/1592599079 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QR |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSG |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI045000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSG |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 579 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Human Retrovirus Protocols |
| Medium | [electronic resource] : |
| Remainder of title | Virology and Molecular Biology / |
| Statement of responsibility, etc. | edited by Tuofu Zhu. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 2005. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Totowa, NJ : |
| Name of producer, publisher, distributor, manufacturer | Humana Press : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2005. |
| 300 ## - online resource | |
| Extent | XIV, 494 p. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 304 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Methods for Virus Isolation and Detection -- Enhanced Culture Assay for Detection and Quantitation of Latently Infected, Resting CD4+ T-Cells Carrying Replication-Competent Virus in HIV-1-Infected Individuals -- Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals -- Isolation of Human Immunodeficiency Virus Type 1 From Peripheral Blood Monocytes -- Isolation, Propagation, and HIV-1 Infection of Monocyte-Derived Macrophages and Recovery of Virus From Brain and Cerebrospinal Fluid -- Isolation and HIV-1 Infection of Primary Human Microglia From Fetal and Adult Tissue -- Isolation of Human Immunodeficiency Virus Type 1 From Semen and Vaginal Fluids -- Isolation and Quantification of HIV From Lymph Nodes -- Isolation of Human Immunodeficiency Virus Type 2 Biological Clones From Peripheral Blood Lymphocytes -- Isolation and Confirmation of Human T-Cell Leukemia Virus Type 2 From Peripheral Blood Mononuclear Cells -- Isolation of Foamy Viruses From Peripheral Blood Lymphocytes -- Alu-LTR Real-Time Nested PCR Assay for Quantifying Integrated HIV-1 DNA -- Quantification of HFV-Integrated DNA in Human Cells by Alu-LTR Real-Time PCR -- Detection of HIV-1 Provirus and RNA by In Situ Amplification -- Detection of HTLV-1 Gene on Cytologic Smear Slides -- Detection of HIV-2 by PCR -- Quantitation of HIV-1 Viral RNA in Blood Plasma and Genital Secretions -- Quantification of Proviral DNA Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real-Time PCR -- Plasma RNA Viral Load in HIV-1 Group O Infection by Real-Time PCR -- A New Combined HIV p24 Antigen and Anti-HIV-1/2/O Screening Assay -- A New Automated Fourth-Generation HIV Screening Assay With Sensitive Antigen Detection Module and High Specificity -- Detection of Antiviral Antibodies Using Enzyme-Linked Immunosorbent Assay -- Plaque-Reduction Assays for Human and Simian Immunodeficiency Virus Neutralization -- Detection of Drug-Resistant HIV-1 Strains -- Molecular Biology Methods -- Determination of Cell Tropism of HIV-1 -- Determination of Co-Receptor Usage of HIV-1 -- Quantitative Evaluation of HIV and SIV Co-Receptor Use With GHOST(3) Cell Assay -- Phenotypic Characterization of Blood Monocytes From HIV-Infected Individuals -- Methods to Determine HIV-1 Ex Vivo Fitness -- A Yeast Recombination-Based Cloning System to Produce Chimeric HIV-1 Viruses and Express HIV-1 Genes -- PCR Amplification, Cloning, and Construction of HIV-1 Infectious Molecular Clones From Virtually Full-Length HIV-1 Genomes -- Amplification and Cloning of Near Full-Length HIV-2 Genomes -- Growth and Manipulation of a Human T-Cell Leukemia Virus Type 2 Full-Length Molecular Clone -- Construction and Analysis of Genomic, Full-Length Infectious Foamy Virus DNA Clones -- Molecular Characterization of Proteolytic Processing of the Gag Proteins of Human Spumaretrovirus -- Assessing the Relative Efficacy of Antiretroviral Activity of Different Drugs on Macrophages -- Gene Expression Profiling of HIV-1 Infection Using cDNA Microarrays -- Determination of DC-SIGN and DC-SIGNR Repeat Region Variations. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Advances in our understanding of human retroviruses have been occurring at a very rapid pace and have contributed significantly to the development of new biotechnologies. In Human Retrovirus Protocols: Virology and Molecular Biology, recognized international researchers describe in detail the basic and state-of-the-art methods they have optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from proven methods for the isolation and detection of human retroviruses to advanced methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from peripheral blood mononuclear cells (PBMC), blood monocytes, brain tissues, cerebrospinal fluids, semen, the vagina, and lymph nodes. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinant virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection. The protocols follow the highly praised Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Human Retrovirus Protocols: Virology and Molecular Biology offers novice and experienced researchers alike proven tools essential for productive study and understanding of the human retroviruses. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Microbiology. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Microbiology. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhu, Tuofu. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781588294951 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781617375996 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 304 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1385/1592599079">https://doi.org/10.1385/1592599079</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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