Argonaute Proteins (Record no. 8519)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04486nam a22004935i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-61779-046-1 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083722.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 110428s2011 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781617790461 |
| -- | 978-1-61779-046-1 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1007/978-1-61779-046-1 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QD431-431.7 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSBC |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI049000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSB |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572.6 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Argonaute Proteins |
| Medium | [electronic resource] : |
| Remainder of title | Methods and Protocols / |
| Statement of responsibility, etc. | edited by Tom C. Hobman, Thomas F. Duchaine. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 2011. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Totowa, NJ : |
| Name of producer, publisher, distributor, manufacturer | Humana Press : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2011. |
| 300 ## - online resource | |
| Extent | XII, 344 p. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 725 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Purification of Native Argonaute Complexes from the Fission Yeast Schizosaccharomyces pombe -- Chromatin Immuno-Precipitation in Fission Yeast -- Biochemical Analyses of Endogenous Argonaute Complexes Immunopurified with Anti-Argonaute Monoclonal Antibodies -- Mapping of Ago2-GW182 Functional Interactions -- Continuous Density Gradients to Study Argonaute and GW182 Complexes Associated with the Endocytic Pathway -- In vitro RISC Cleavage Assay -- Native Gel Analysis for RISC Assembly -- Purification and Assembly of Human Argonaute, Dicer, and TRBP Complexes -- Detection of Human Dicer and Argonaute 2 Catalytic Activity -- Imaging the Cellular Dynamics of Drosophila Argonaute Proteins -- Live Cell Imaging of Argonaute Proteins in Mammalian Cells -- Reporter-Based Assays for Analyzing RNA Interference in Mammalian Cells -- Artificial Tethering of Argonaute Proteins for Studying their Role in Translational Repression of Target mRNAs -- An Efficient System For Let-7 MicroRNA- And GW182 Protein-Mediated Deadenylation In vitro -- Cell-Free microRNA-Mediated Translation Repression in C. elegans -- Argonaute Pull-Down and RISC Analysis Using 2'-O-Methylated Oligonucleotides Affinity Matrices -- Cloning Argonaute-Associated Small RNAs from C. elegans -- Immunoprecipitation of piRNPs and Directional, Next Generation Sequencing of piRNAs -- Generation of an Inducible Mouse ES Cell Line Deficient for Argonaute Proteins -- Whole Cell Proteome Regulation by microRNAs Captured in a Pulsed SILAC Mass Spectrometry Approach. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner. Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels. In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Proteins . |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Biotechnology. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Protein Biochemistry. |
| 650 24 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Chemical Bioengineering. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hobman, Tom C. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Duchaine, Thomas F. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781617790454 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781617790478 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781493958276 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 725 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1007/978-1-61779-046-1">https://doi.org/10.1007/978-1-61779-046-1</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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