Immunocytochemical Methods and Protocols (Record no. 8491)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 05811nam a22004335i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-59259-213-5 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083722.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 100301s1999 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781592592135 |
| -- | 978-1-59259-213-5 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1385/1592592139 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QD415-436 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSB |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI007000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSB |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Immunocytochemical Methods and Protocols |
| Medium | [electronic resource] / |
| Statement of responsibility, etc. | edited by Lorette C. Javois. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 2nd ed. 1999. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Totowa, NJ : |
| Name of producer, publisher, distributor, manufacturer | Humana Press : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 1999. |
| 300 ## - online resource | |
| Extent | 465 p. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 115 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Antibody Preparation -- Overview of Antibody Use in Immunocytochemistry -- Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration -- Purification of Antibodies Using Ion-Exchange Chromatography -- Purification of Antibodies Using Affinity Chromatography -- Purification of Antibodies Using Protein A-Sepharose and FPLC -- Conjugation of Fluorochromes to Antibodies -- Biotinylation of Antibodies -- Tissue Preparation for Light Microscopic Analyses -- Overview of Cell Fixatives and Cell Membrane Permeants -- Preparation of Frozen Sections for Analysis -- Processing of Cytological Specimens -- Processing of Tissue-Culture Cells -- Processing of Tissue Specimens -- Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections -- Light Microscopic Detection Systems -- Overview of Fluorochromes -- Direct Immunofluorescent Labeling of Cells -- Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells -- Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells -- Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts -- TUNEL Assay for Apoptotic Cells -- Overview of Fluorescence Analysis with the Confocal Microscope -- Overview of Laser Microbeam Applications as Related to Antibody Targeting -- Overview of Fluorescence Photomicrography -- Overview of Antigen Detection Through Enzymatic Activity -- The Peroxidase-Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods -- The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods -- Avidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue -- Multiple Antigen Immunostaining Procedures -- ELISA on Attachment-Dependent or Suspension Grown Cells -- Use of Immunogold with Silver Enhancement -- Fluorescence Activated Cell Sorter (FACS)Analyses -- Overview of Flow Cytometry and Fluorescent Probes for Cytometry -- Tissue Disaggregation -- Indirect Immunofluorescent Labeling ofViable Cells -- Indirect Immunofluorescent Labeling of Fixed Cells -- Fluorescent Labeling of DNA -- Deparaffinization and Processing of Pathologic Material -- Assay for Phagocytosis -- Assay for Filamentous Actin -- Assay for Chemoattractant Binding -- Assay for Oxidative Metabolism -- Colloidal Gold Detection Systems for Electron Microscopic Analyses -- Fixation and Embedding -- Preparation of Colloidal Gold -- Conjugation of Colloidal Gold to Proteins -- Colloidal Gold/Streptavidin Methods -- Pre-Embedding Labeling Methods -- Postembedding Labeling Methods -- Applications for Nucleic Acid Localization by In Situ Hybridization -- Overview of In Situ Hybridization -- In Situ Hybridization to Human Chromosomes of an Alkaline Phosphatase-Labeled Centromeric Probe -- Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes -- In Situ RT-PCR and Hybridization Techniques -- Special Applications for the Clinical Laboratory -- Overview of the Clinical Immunohistochemistry Laboratory: Regulationsand Troubleshooting Guidelines -- Overview of Immunocytochemical Approaches to the Differential Diagnosis of Tumors -- Overview of Automated Immunostainers. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | In Animal Cell Biotechnology, scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large- scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection; cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture. This authoritative book will significantly aid researchers in developing new monoclonal antibodies, vaccines, anticancer agents, and drugs for the widest spectrum of cardiovascular, respiratory, and immune diseases. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Biochemistry. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Biochemistry. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Javois, Lorette C. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9780896038134 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 115 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1385/1592592139">https://doi.org/10.1385/1592592139</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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