Clostridium difficile (Record no. 8125)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04392nam a22004815i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-4939-6361-4 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083715.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160809s2016 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781493963614 |
| -- | 978-1-4939-6361-4 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1007/978-1-4939-6361-4 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QR180-189.5 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | MJCM |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | MED044000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | MJCM |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 571.96 |
| Edition information | 23 |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 616.079 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Clostridium difficile |
| Medium | [electronic resource] : |
| Remainder of title | Methods and Protocols / |
| Statement of responsibility, etc. | edited by Adam P. Roberts, Peter Mullany. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 2nd ed. 2016. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | New York, NY : |
| Name of producer, publisher, distributor, manufacturer | Springer New York : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2016. |
| 300 ## - online resource | |
| Extent | XI, 292 p. 49 illus., 33 illus. in color. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 1476 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Restriction Endonuclease Analysis Typing of Clostridium Difficile Isolates -- Direct PCR-ribotyping of Clostridium difficile -- From FASTQ to Function: In silico Methods for Processing Next Generation Sequencing Data -- Clostridium difficile Genome Editing Using pyrE Alleles -- Use of mCherryOpt Fluorescent Protein in Clostridium difficile -- A Fluorescent Reporter for Single Cell Analysis of Gene Expression in Clostridium difficile -- Clostridium difficile Adhesins -- Intestinal Epithelial Cell Response to Clostridium difficile Flagella -- Isolating and Purifying Clostridium difficile Spores -- Inducing and Quantifying Clostridium difficile Spore Formation -- Characterization of Functional Prophages in Clostridium difficile -- Induction and Purification of C. difficile Phage Tail-like Particles -- Phage Transduction -- Transfer of Clostridium difficile Genetic Elements Conferring Resistance to Macrolide-Lincosamide-streptograminB (MLSB) Antibiotics -- Methods for Determining Transfer of Mobile Genetic Elements in Clostridium difficile -- Investigating Transfer of Large Chromosomal Regions Containing the Pathogenicity Locus between Clostridium difficile Strains -- An in vitro Model of the Human Colon: Studies of Intestinal Biofilms and lostridium difficile Infection -- MiniBioReactor Arrays (MBRAs) as a Tool for Studying C. difficile Physiology in the Presence of a Complex Community -- A Practical Method for Preparation of Fecal Microbiota Transplantation -- Ion Exchange Chromatography to Analyze Components of a Clostridium difficile Vaccine -- A Size Exclusion Chromatography Method for Analysis of Clostridium difficile Vaccine Toxins. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This second edition provides 21 new chapters on methods used in laboratories for investigating the physiology and molecular genetics of the pathogen Clostridium difficile. Chapters detail up-to -date experimental techniques for gene editing and transcriptional analysis which are used to investigate the fundamental biology of the organism and its virulence factors. Additional chapters describe development of potential new treatments including vaccines, bacteriophage and faecal transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols, Second Edition provides a comprehensivecatalogue of molecular tools and techniques authored by the researchers who have developed them. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Immunology. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Immunology. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Roberts, Adam P. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mullany, Peter. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781493963591 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781493963607 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781493981762 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 1476 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1007/978-1-4939-6361-4">https://doi.org/10.1007/978-1-4939-6361-4</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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