Genomic Imprinting (Record no. 7995)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04663nam a22004575i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-59259-211-1 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083713.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 100301s2002 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781592592111 |
| -- | 978-1-59259-211-1 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1385/1592592112 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | RB155-155.8 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | MFN |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | MED107000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | MFN |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 616.042 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Genomic Imprinting |
| Medium | [electronic resource] : |
| Remainder of title | Methods and Protocols / |
| Statement of responsibility, etc. | edited by Andrew Ward. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 2002. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Totowa, NJ : |
| Name of producer, publisher, distributor, manufacturer | Humana Press : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2002. |
| 300 ## - online resource | |
| Extent | XII, 377 p. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 181 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Generation of Monoparental Embryos for Investigation into Genomic Imprinting -- Deriving and Propagating Mouse Embryonic Stem Cell Lines for Studying Genomic Imprinting -- Balanced Translocations for the Analysis of Imprinted Regions of the Mouse Genome -- Production of YAC Transgenic Mice by Pronuclear Injection -- A Transgenic Approach to Studying Imprinted Genes -- Methylation-Sensitive Genome Scanning -- Subtraction-Hybridization Method for the Identification of Imprinted Genes -- Identification of Imprinted Loci by Methylation -- Ribonuclease Protection -- Quantitative RT-PCR-Based Analysis of Allele-Specific Gene Expression -- Allele-Specific In Situ Hybridization (ASISH) -- RNA-FISH to Analyze Allele-Specific Expression -- Flow Cytometry and FISH to Investigate Allele-Specific Replication Timing and Homologous Association of Imprinted Chromosomes -- Southern Analysis Using Methyl-Sensitive Restriction Enzymes -- A PCR-Based Method for Studying DNA Methylation -- Bisulfite-Based Methylation Analysis of Imprinted Genes -- Direct Analysis of Chromosome Methylation -- In Vitro Methylation of Predetermined Regions in Recombinant DNA Constructs -- In Vitro Methylation of Specific Regions in Recombinant DNA Constructs by Excision and Religation -- Detection of Methyl?Sensitive DNA?Binding Proteins with Possible Involvement in the Imprinting Phenomenon -- Probing Chromatin Structure with Nuclease Sensitivity Assays -- Examining Histone Acetlylation at Specific Genomic Regions -- Purification of the MeCP2/Histone Deacetylase Complex from Xenopus laevis -- Reconstitution of Chromatin In Vitro -- Genomic Imprinting in Plants. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Imprinted genes, many of which generally control growth and development, frequently lose their imprints during cancer progression, a loss that then plays a substantial role in uncontrolled tumor growth. Imprint instability also appears to be a major limitation to the success of mammalian cloning experiments. In Genomic Imprinting: Methods and Protocols, Andrew Ward and a team of experienced researchers have brought together a collection of optimized classic and vanguard techniques for the identification and analysis of imprinted genes. The majority of protocols describe molecular techniques that allow examination of gene structure or expression in an allele-specific manner. Protocols are included for identifying and cloning imprinted genes, for analyzing imprinted gene expression, for the study of DNA methylation and methylation-sensitive DNA-binding proteins, and for examining chromatin structure. There are also methods for the manipulation of mouse embryos to produce monoparental embryos and embryonic stem cells, and for the generation of transgenic mice with BAC, PAC, and YAC constructs. Each technique is described in step-by-step detail to ensure successful results. Incorporating a wealth of knowledge from leading exponents in the field, Genomic Imprinting: Methods and Protocols brings together all the essential molecular, genetic, and embryological methods commonly used in today's laboratories for the identification and analysis of imprinted genes. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Medical genetics. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Medical Genetics. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ward, Andrew. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9780896037410 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781617371646 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781489938954 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 181 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1385/1592592112">https://doi.org/10.1385/1592592112</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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