In Vitro Mutagenesis Protocols (Record no. 7936)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 06044nam a22005055i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-60761-652-8 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083712.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 100803s2010 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781607616528 |
| -- | 978-1-60761-652-8 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1007/978-1-60761-652-8 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | TP248.13-.65 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | TCB |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | TEC009010 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | TCB |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.63 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | In Vitro Mutagenesis Protocols |
| Medium | [electronic resource] : |
| Remainder of title | Third Edition / |
| Statement of responsibility, etc. | edited by Jeff Braman. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 3rd ed. 2010. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Totowa, NJ : |
| Name of producer, publisher, distributor, manufacturer | Humana Press : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2010. |
| 300 ## - online resource | |
| Extent | XIV, 442 p. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 634 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Mutagenesis in Various Microbial Backgrounds -- Mutagenesis Protocols in Saccharomyces cerevisiae by In Vivo Overlap Extension -- In Vitro Mutagenesis of Brucella Species -- Random Mutagenesis Strategies for Campylobacter and Helicobacter Species -- Mutagenesis of the Repeat Regions of Herpesviruses Cloned as Bacterial Artificial Chromosomes -- An Efficient Protocol for VZV BAC-Based Mutagenesis -- A Method for Rapid Genetic Integration into Plasmodium falciparum Utilizing Mycobacteriophage Bxb1 Integrase -- PCR Mutagenesis -- Random Mutagenesis by Error-Prone PCR -- A Rapid and Versatile PCR-Based Site-Directed Mutagenesis Protocol for Generation of Mutations Along the Entire Length of a Cloned cDNA -- Rapid Sequence Scanning Mutagenesis Using In Silico Oligo Design and the Megaprimer PCR of Whole Plasmid Method (MegaWHOP) -- Insertion and Deletion Mutagenesis by Overlap Extension PCR -- Targeted Amplification of Mutant Strands for Efficient Site-Directed Mutagenesis and Mutant Screening -- A Modified Inverse PCR Procedure for Insertion, Deletion, or Replacement of a DNA Fragment in a Target Sequence and Its Application in the Ligand Interaction Scan Method for Generation of Ligand-Regulated Proteins -- Amplification of Orthologous Genes Using Degenerate Primers -- Reviews -- Computational Evaluation of Protein Stability Change upon Mutations -- Approaches for Using Animal Models to Identify Loci That Genetically Interact with Human Disease-Causing Point Mutations -- Protein Evolution Mutagenesis -- Using Peptide Loop Insertion Mutagenesis for the Evolution of Proteins -- Massive Mutagenesis®: High-Throughput Combinatorial Site-Directed Mutagenesis -- Directed In Vitro Evolution of Reporter Genes Based on Semi-Rational Design and High-Throughput Screening -- Ribosome Display for RapidProtein Evolution by Consecutive Rounds of Mutation and Selection -- Protein Structure and Function Mutagenesis -- Fine-Tuning Enzyme Activity Through Saturation Mutagenesis -- Characterization of Structural Determinants of Type 1 Corticotropin Releasing Hormone (CRH) Receptor Signalling Properties -- Site-Directed Mutagenesis for Improving Biophysical Properties of VH Domains -- Phenotype Based Functional Gene Screening Using Retrovirus-Mediated Gene Trapping in Quasi-Haploid RAW 264.7 Cells -- Site-Directed Disulfide Cross-Linking to Probe Conformational Changes of a Transporter During Its Functional Cycle: Escherichia coli AcrB Multidrug Exporter as an Example -- Site-Specific Incorporation of Extra Components into RNA by Transcription Using Unnatural Base Pair Systems -- Random Mutagenesis -- Mutagen™: A Random Mutagenesis Method Providing a Complementary Diversity Generated by Human Error-Prone DNA Polymerases -- Random-Scanning Mutagenesis -- Easy Two-Step Method for Randomizing and Cloning Gene Fragments -- Mutator Bacterial Strain Mutagenesis -- Random Mutagenesis Using a Mutator Strain -- En Passant Mutagenesis: A Two Step Markerless Red Recombination System. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Biotechnology. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Medical genetics. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Proteins . |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Chemical Bioengineering. |
| 650 24 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Medical Genetics. |
| 650 24 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Protein Biochemistry. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Braman, Jeff. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781607616511 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781607616610 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781493956661 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 634 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1007/978-1-60761-652-8">https://doi.org/10.1007/978-1-60761-652-8</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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