GHU Logo
The Great Heights University Library
Academic discovery, digital access, and research support

Molecular Diagnosis of Infectious Diseases (Record no. 7933)

MARC details
000 -LEADER
fixed length control field 05202nam a22004695i 4500
001 - CONTROL NUMBER
control field 978-1-59259-679-9
003 - CONTROL NUMBER IDENTIFIER
control field DE-He213
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20260610083712.0
007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION
fixed length control field cr nn 008mamaa
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 100301s2004 xxu| s |||| 0|eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 9781592596799
-- 978-1-59259-679-9
024 7# - OTHER STANDARD IDENTIFIER
Standard number or code 10.1385/1592596797
Source of number or code doi
050 #4 - LIBRARY OF CONGRESS CALL NUMBER
Classification number RC1-1245
072 #7 - SUBJECT CATEGORY CODE
Subject category code MJC
Source bicssc
072 #7 - SUBJECT CATEGORY CODE
Subject category code MED022000
Source bisacsh
072 #7 - SUBJECT CATEGORY CODE
Subject category code MJC
Source thema
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 616
Edition information 23
245 10 - TITLE STATEMENT
Title Molecular Diagnosis of Infectious Diseases
Medium [electronic resource] /
Statement of responsibility, etc. edited by Jochen Decker, Udo Reischl.
250 ## - EDITION STATEMENT
Edition statement 2nd ed. 2004.
264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture Totowa, NJ :
Name of producer, publisher, distributor, manufacturer Humana Press :
-- Imprint: Humana,
Date of production, publication, distribution, manufacture, or copyright notice 2004.
300 ## - online resource
Extent XVI, 464 p.
Other physical details online resource.
336 ## - CONTENT TYPE
Content type term text
Content type code txt
Source rdacontent
337 ## - MEDIA TYPE
Media type term computer
Media type code c
Source rdamedia
338 ## - CARRIER TYPE
Carrier type term online resource
Carrier type code cr
Source rdacarrier
347 ## - DIGITAL FILE CHARACTERISTICS
File type text file
Encoding format PDF
Source rda
490 1# - SERIES STATEMENT
Series statement Methods in Molecular Medicine,
International Standard Serial Number 1940-6037 ;
Volume/sequential designation 94
505 0# - FORMATTED CONTENTS NOTE
Formatted contents note Protein-Based Identification Of Antigens -- Proteomic Approaches to Antigen Discovery -- Immunoproteomics -- Immunoprecipitation and Blotting -- DNA-Based Identification of Antigens -- Representational Difference Analysis of cDNA -- Microarray Data Analysis and Mining -- Expression Cloning -- Identification of Epitopes and Immunomodulatory Components -- Determination of Epitopes by Mass Spectrometry -- Identification of T-Cell Epitopes Using ELISpot and Peptide Pool Arrays -- Virus-like Particles -- Application of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components -- Expression of Recombinant Proteins -- High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format -- Production of Antigens in Chlamydomonas reinhardtii -- Codon-Optimized Genes that Enable Increased Heterologous Expression in Mammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNA -- Purification, Modification, and Renaturation of Recombinant Proteins -- Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies) -- Purification of Recombinant Proteins with High Isoelectric Points -- Refolding of Inclusion Body Proteins -- Small-Molecule-Protein Conjugation Procedures -- Characterization of Recombinant Proteins -- Structural Characterization of Proteins and Peptides -- Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors -- Affinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) Biosensor -- Evaluation of Recombinant Proteins in Immunological Test Systems -- Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays -- Design and Preparationof Recombinant Antigens as Diagnostic Reagents in Solid-Phase Immunosorbent Assays -- Basic Problems of Serological Laboratory Diagnosis -- Molecular Diagnostics Resources on the Internet -- Recombinant Receptor Molecules -- Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells.
520 ## - SUMMARY, ETC.
Summary, etc. In this second edition of a much-acclaimed classic laboratory manual, Molecular Diagnosis of Infectious Diseases, leading experts from academia and industry describe cutting-edge methods for the protein-based diagnosis of infectious diseases. Explaining the latest developments in genomics, proteomics, bioinformatics, biosensors, high-throughput devices, and recombinant technology, the authors demonstrate the power of these new methodologies in both established and novel procedures by applying them successfully to the identification and characterization of valuable diagnostic markers, immunomodulatory components, epitope mapping, the production and purification of recombinant antigens, and their use in diagnostic reagents in immunological assays. Basic problems of serological laboratory diagnostics and the use of immunoproteomics for discovering target proteins are also addressed. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and extensive references. Comprehensive and up-to-date, Molecular Diagnosis of Infectious Diseases, Second Edition offers novice investigators an easy entry into the field and experienced researchers a broad array of time saving tools that will not only elucidate new principles, but also greatly facilitate the development and successful application of rapid diagnostic methods.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Diseases.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Diseases.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Decker, Jochen.
Relator term editor.
Relationship edt
-- http://id.loc.gov/vocabulary/relators/edt
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Reischl, Udo.
Relator term editor.
Relationship edt
-- http://id.loc.gov/vocabulary/relators/edt
710 2# - ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element SpringerLink (Online service)
773 0# - HOST ITEM ENTRY
Title Springer Nature eBook
776 08 - ADDITIONAL PHYSICAL FORM ENTRY
Relationship information Printed edition:
International Standard Book Number 9781588292216
776 08 - ADDITIONAL PHYSICAL FORM ENTRY
Relationship information Printed edition:
International Standard Book Number 9781617374302
776 08 - ADDITIONAL PHYSICAL FORM ENTRY
Relationship information Printed edition:
International Standard Book Number 9781489939548
830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE
Uniform title Methods in Molecular Medicine,
International Standard Serial Number 1940-6037 ;
Volume/sequential designation 94
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://doi.org/10.1385/1592596797">https://doi.org/10.1385/1592596797</a>
912 ## -
-- ZDB-2-PRO

No items available.