Drosophila Cytogenetics Protocols (Record no. 7736)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04550nam a22004575i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-59259-665-2 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083708.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 100301s2004 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781592596652 |
| -- | 978-1-59259-665-2 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1385/1592596657 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QH426-470 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSAK |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI029000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSAK |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 576.5 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Drosophila Cytogenetics Protocols |
| Medium | [electronic resource] / |
| Statement of responsibility, etc. | edited by Daryl S. Henderson. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 2004. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Totowa, NJ : |
| Name of producer, publisher, distributor, manufacturer | Humana Press : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2004. |
| 300 ## - online resource | |
| Extent | XIII, 468 p. 269 illus., 30 illus. in color. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 247 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | The Chromosomes of Drosophila melanogaster -- Spermatogenesis -- Time-Lapse Imaging of Male Meiosis by Phase-Contrast and Fluorescence Microscopy -- Immunocytological Analysis of Oogenesis -- Cytological Analysis of Oogenesis -- Polytene Chromosomes From Ovarian Nurse Cells of Drosophila melanogaster otu Mutants -- Feulgen-DNA Cytophotometry for Estimating C Values -- Fluorescent BrdU Labeling and Nuclear Flow Sorting of the Drosophila Ovary -- Studying Nuclear Organization in Embryos Using Antibody Tools -- Whole-Mount Fluorescence In Situ Hybridization to Chromosomes of Embryos -- Orcein Staining and the Identification of Polytene Chromosomes -- Salivary Chromosome Analysis of Aberrations -- In Situ Hybridization to Polytene Chromosomes -- Combined Immunostaining and FISH Analysis of Polytene Chromosomes -- Electron Microscopy of Polytene Chromosomes -- Analysis of Mitosis in Squash Preparations of Larval Brains -- Analyzing Chromosome Function by High Frequency Formation of Dicentric Chromosomes In Vivo -- Analysis of Chromosomes of the Larval CNS by FISH and BrdU Labeling -- Immunostaining of Squash Preparations of Chromosomes of Larval Brains -- Visualizing Mitosis in Whole-Mount Larval Brains -- Immunostaining of Whole-Mount Imaginal Discs -- Wing Somatic Mutation and Recombination Test -- Analysis of Histoblasts -- Visualizing Apoptosis -- RNAi in Cultured Drosophila Cells. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Probably no single experimental organism has contributed as much to our understanding of the structure, function, and mechanism of transmission of eukaryotic chromosomes as has the fruit fly, Drosophila melanogaster. In Drosophila Cytogenetics Protocols, leading drosophilists describe, in step-by-step detail, all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. General areas covered include analyses of nuclei and chromosomes in spermatogenesis and oogenesis, fluorescence-activated cell sorting of ovarian follicle cells, analyses of nuclear division cycles in embryos, and analyses of programmed cell death. In addition, there are conventional light-, electron-, and immunofluorescence-microscopic analyses of polytene chromosomes; cytological analyses of larval brains, imaginal discs, and histoblasts; and nonfluorescent and fluorescent in situ hybridization (FISH) to both polytene and mitotic chromosomes. A cutting-edge combined FISH and immunolocalization protocol will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression. Each readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. Readily accessible and highly practical, Drosophila Cytogenetics Protocols provides a comprehensive cytogenetics laboratory manual for Drosophila investigators, one that is suitable not only for novices, but also highly informative for seasoned investigators. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Genetics. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Genetics and Genomics. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Henderson, Daryl S. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781588290502 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781617373251 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781489928153 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 247 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1385/1592596657">https://doi.org/10.1385/1592596657</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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