Molecular Methods in Developmental Biology (Record no. 7734)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03799nam a22004575i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-59259-678-2 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083708.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 100301s1999 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781592596782 |
| -- | 978-1-59259-678-2 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1385/1592596789 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QH491-492 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSC |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI072000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSC |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 571.8 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Molecular Methods in Developmental Biology |
| Medium | [electronic resource] : |
| Remainder of title | Xenopus and Zebrafish / |
| Statement of responsibility, etc. | edited by Matt Guille. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 1999. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Totowa, NJ : |
| Name of producer, publisher, distributor, manufacturer | Humana Press : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 1999. |
| 300 ## - online resource | |
| Extent | XII, 217 p. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 127 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | The Animal Cap Assay -- Cell and Tissue Transplantation in Zebrafish Embryos -- Ribonuclease Protection Analysis of Gene Expression in Xenopus -- Quantitative Analysis of mRNA Levels in Xenopus Embryos by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) -- Wholemount In Situ Hybridization of Xenopus and Zebrafish Embryos -- In Situ Hybridization to Sections of Xenopus Embryos -- Zebrafish Immunohistochemistry -- Immunohistochemistry of Xenopus Embryos -- Preparation and Testing of Synthetic mRNA for Microinjection -- Microinjection into Xenopus Oocytes and Embryos -- Microinjection into Zebrafish Embryos -- Expression from DNA Injected into Xenopus Embryos -- Promoter Analysis in Zebrafish Embryos -- Transient Transgenesis in Xenopus laevis Facilitated by AAV-ITRs -- Band-Shift Analysis Using Crude Oocyte and Embryo Extracts from Xenopus laevis -- DNA-Footprinting using Crude Embryonic Extracts from Xenopus laevis -- Mapping Protein-DNA Interactions Using In Vivo Footprinting. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | In Molecular Methods in Developmental Biology: Xenopus and Zebrafish, Matthew Guille assembles a hands-on collection of basic and essential molecular and embryological techniques for studying Xenopus and zebrafish. Easily reproducible and designed to succeed, these detailed methods include cellular techniques, techniques for the quantitative and spatial analysis of mRNA and proteins, and techniques for the expression of gene products in embryos. More specialized methods enable users to analyze promoters and transcription factors during early development, and include gel shift assays, as well as in vitro and in vivo footprinting. Wherever possible, these experimental approaches are applied to both Xenopus and zebrafish. Molecular Methods in Developmental Biology: Xenopus and Zebrafish affords newcomers rapid access to a wide variety of key techniques in developmental research, and offers experienced investigators both new techniques from experts who have fine-tuned them for best results, and a plethora of time-saving tips. State-of-the-art and readily reproducible, these powerful methods constitute today's gold-standard laboratory manual for understanding the interactions responsible for development. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Developmental biology. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Developmental Biology and Stem Cells. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Guille, Matt. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9780896037908 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781617371899 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781489943316 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 127 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1385/1592596789">https://doi.org/10.1385/1592596789</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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