Chromatin Protocols (Record no. 7578)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 05523nam a22004575i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-4939-2474-5 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083706.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 150331s2015 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781493924745 |
| -- | 978-1-4939-2474-5 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1007/978-1-4939-2474-5 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QH573-671 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSF |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI017000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSF |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 571.6 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Chromatin Protocols |
| Medium | [electronic resource] / |
| Statement of responsibility, etc. | edited by Srikumar P. Chellappan. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 3rd ed. 2015. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | New York, NY : |
| Name of producer, publisher, distributor, manufacturer | Springer New York : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2015. |
| 300 ## - online resource | |
| Extent | XIV, 492 p. 74 illus., 36 illus. in color. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 1288 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Beads-on-a-String on a Bead: Reconstitution and Analysis of Chromatin on a Solid Support -- Preparation and Analysis of Positioned Mononucleosomes -- Chromatin Imaging with Time-Lapse Atomic Force Microscopy -- Isolation of Specific Genomic Regions and Identification of Associated Molecules by Engineered DNA-Binding Molecule-Mediated Chromatin Immunoprecipitation (enChIP) Using CRISPR -- Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin -- Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes -- Histone Deacetylase Activity Assay -- In Vitro Histone Demethylase Assays -- Integrated DNA Methylation and Chromatin Structural Analysis at Single-Molecule Resolution -- Determination of DNA Methylation Levels Using Illumina HumanMethylation450 BeadChips -- Investigation of Genomic Methylation Status Using Methylation Specific and Bisulfite Sequencing Polymerase Chain Reaction -- In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination -- Immunostaining Analysis of Tissue Cultured Cells and Tissue Sections Using Phospho-Histone H3 (Serine 10) Antibody -- Identification and Characterization of Nonhistone Chromatin Proteins: Human Positive Coactivator 4 as a Candidate -- Methods to Study Transcription-Coupled Repair in Chromatin -- Analysis of DNA Replication Associated Chromatin Decondensation: In Vivo Assay for Understanding Chromatin Remodeling Mechanisms of Selected Proteins -- Non-Radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length -- Detecting ATM Dependent Chromatin Modification in DNA Damage Response -- Imaging Local Deposition of Newly Synthesized Histones in UVC-Damaged Chromatin -- In Vitro Replication Assay with Mammalian Cell Extracts -- Fluorescent In Situ Hybridization on Comets: FISH Comet -- Methods to Study Histone Chaperone Function in Nucleosome Assembly and Chromatin Transcription -- Preparation of Mononucleosomal Templates for Analysis of Transcription with RNA Polymerase Using spFRET -- Transcriptome-Wide Identification of In Vivo Interactions Between RNAs and RNA-Binding Proteins by RIP and PAR-CLIP Assays -- Chromatin Immunoprecipitation Assays: Analyzing Transcription Factor Binding and Histone Modifications In Vivo -- ChIP on Chip and ChIP-Seq Assays: Genome-Wide Analysis of Transcription Factor Binding and Histone Modifications -- ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Updated and revised, this thorough volume is organized such that it begins with techniques related to the study of chromatin structure. Protocols for reconstitution of chromatin on solid supports for analysis, preparation of positioned mononucleosomes, techniques to study premature chromatin condensation, and the use of comparative genomic hybridization to assess genomic aberration are included as well. Novel techniques for imaging chromatin using atomic force microscopy and the isolation of specific genomic regions using engineered DNA binding molecules generated by CRISPR are then examined. That section is followed by protocols to analyze DNA and histone modifications, while the third section includes methods to study DNA replication and repair, in the context of chromatin. Last but not least, protocols for studying chromatin and its relation with transcriptional regulation are presented in a fourth section. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Chromatin Protocols, Third Edition aims to help researchers in facilitating in-depth molecular analysis of various aspects of chromatin structure and function. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Cytology. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Cell Biology. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chellappan, Srikumar P. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781493924738 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781493924752 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781493944064 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 1288 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1007/978-1-4939-2474-5">https://doi.org/10.1007/978-1-4939-2474-5</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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