Bone Research Protocols (Record no. 7430)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04671nam a22004695i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-59259-366-8 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083703.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 100301s2003 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781592593668 |
| -- | 978-1-59259-366-8 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1385/1592593666 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | RD701-811 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | MNS |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | MED065000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | MNS |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 616.7 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Bone Research Protocols |
| Medium | [electronic resource] / |
| Statement of responsibility, etc. | edited by Miep H. Helfrich, Stuart H. Ralston. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 2003. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Totowa, NJ : |
| Name of producer, publisher, distributor, manufacturer | Humana Press : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2003. |
| 300 ## - online resource | |
| Extent | XIV, 450 p. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Medicine, |
| International Standard Serial Number | 1940-6037 ; |
| Volume/sequential designation | 80 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Culture of Osteolblasts and Osteocytes -- Human Osteoblast Culture -- Osteoblast Isolation from Murine Calvariae and Long Bones -- Mineralizing Fibroblast-Colony-Forming Assays -- Osteocyte Isolation and Culture -- Culture of Osteoclasts -- Isolated Osteoclast Cultures -- Primary Isolation and Culture of Chicken Osteoclasts -- Isolation and Purification of Rabbit Osteoclasts -- Generating Human Osteoclasts from Peripheral Blood -- Generating Human Osteoclasts In Vitro from Bone Marrow and Peripheral Blood -- Generating Murine Osteoclasts from Bone Marrow -- Osteoclast Formation in the Mouse Coculture Assay -- RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 Cells -- Bone Organ Culture -- Analysis of Osteoclast Function in Mouse Calvarial Cultures -- Assessing Bone Formation Using Mouse Calvarial Organ Cultures -- Histological and Ultrastructural Techniques -- In Situ Hybridization and In Situ Reverse Transcription Polymerase Chain Reaction in Human Bone Sections -- Techniques for the Study of Apoptosis in Bone -- Protein Localization in Wax-Embedded and Frozen Sections of Bone Using Immunohistochemistry -- Detection of Noncollagenous Bone Proteins in Methylmethacrylate-Embedded Human Bone Sections -- Fluorescence Imaging of Bone-Resorbing Osteoclasts by Confocal Microscopy -- Bone Histomorphometry -- Transmission Electron Microscopy of Bone -- Scanning Electron Microscopy of Bone -- Imaging Techniques -- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents -- In Vivo Techniques -- Studies of Local Bone Remodeling -- Inflammation-Induced Osteoporosis -- Ovariectomy and Estrogen Replacement in Rodents -- Mechanical Loading Techniques -- Mechanical Testing of Bone Ex Vivo -- Bone Cell Responses to Fluid Flow -- Methods for Analyzing Bone Cell Responses to Mechanical Loading Using In Vitro Monolayer and Organ Culture Models -- Molecular Analysis -- Extraction of Nucleic Acids from Bone -- Analysis of Gene Expression in Bone by Quantitative RT-PCR. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The past decade has witnessed the discovery of many genes with profound effects on bone. In Bone Research Protocols, recognized authorities with extensive hands-on experience present a wide-ranging catalog of laboratory techniques that will help researchers to investigate the mechanisms by which these genes affect bone mass and strength. Described in step-by-step detail, these readily reproducible methods cover such topics as the isolation and culture of bone cells, the preparation of bone tissue for histological and ultrastructural analysis, methods for the measurement of bone strength and for mechanical studies, and how to use digital imaging techniques in the analysis of bone. Though in vitro methods receive central attention, there are also protocols for in vivo analyses of bone formation and resorption. State-of-the-art and highly practical, Bone Research Protocols offers both beginning and advanced laboratory researchers a major compendium of well-established techniques for studying bone cell function and metabolism. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Orthopedics. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Orthopaedics. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Helfrich, Miep H. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ralston, Stuart H. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781588290441 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781617373213 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781489938527 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Medicine, |
| International Standard Serial Number | 1940-6037 ; |
| Volume/sequential designation | 80 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1385/1592593666">https://doi.org/10.1385/1592593666</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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