Cytoskeleton Methods and Protocols (Record no. 7428)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04692nam a22004575i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-59259-051-3 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083703.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 100301s2001 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781592590513 |
| -- | 978-1-59259-051-3 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1385/1592590519 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QH573-671 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSF |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI017000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSF |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 571.6 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Cytoskeleton Methods and Protocols |
| Medium | [electronic resource] / |
| Statement of responsibility, etc. | edited by Ray H. Gavin. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 2001. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Totowa, NJ : |
| Name of producer, publisher, distributor, manufacturer | Humana Press : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2001. |
| 300 ## - online resource | |
| Extent | XIV, 288 p. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 161 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Identification of Cytoskeleton Proteins -- Using an Inverse PCR Strategy to Clone Large, Contiguous Genomic DNA Fragments -- Microsequencing of Myosins for PCR Primer Design -- Evaluating the Dynein Heavy Chain Gene Family in Tetrahymena -- Microscopy Applications -- Video-Enhanced Microscopy for Analysis of Cytoskeleton Structure and Function -- Computer-Assisted Systems for the Analysis of Amoeboid Cell Motility -- Evaluation of Individual-Cell Motility -- Evaluation of Cell Morphology -- A Quantitative Assay for Measurement of Chemokinesis in Tetrahymena -- Reagents for Studying Cytoskeleton Protein Function -- Jasplakinolide -- Site-Directed Antibodies as Tools for Investigating Structure and Function of Cytoskeleton Proteins -- Direct Labeling of Components in Protein Complexes by Immuno-Electron Microscopy -- Tat-Mediated Delivery of Antibodies into Cultured Cells -- Cytoskeleton Dynamics -- Studying Cytoskeletal Dynamics in Living Cells Using Green Fluorescent Protein -- Use of Green Fluorescent Protein (GFP) to Study Cellular Dynamics -- Transient Transfections and Heterokaryons as Tools for the Analysis of Keratin IF Dynamics -- Cellular Systems as Tools for Investigating Cytoskeleton Structure and Function -- Chromatophores as Tools for the Study of Organelle Transport -- Centriole Duplication, Centrosome Maturation and Spindle Assembly in Lysates of Spisula solidissima Oocytes -- XenopusEgg Extracts as a Model System for Analysis of Microtubule, Actin Filament, and Intermediate Filament Interactions -- Detergent-Extracted Models for the Study of Cilia or Flagella -- Genetic Approaches for Studies of Cytoskeleton Protein Function -- A Yeast Two-Hybrid Approach for Probing Cytoskeletal Protein Interactions -- Manipulating Dynein Genes in Tetrahymena -- Functional Analysis of CytoskeletalComponents in the Developing Visual System of Drosophila. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Many recent advances in experimental instrumentation, reagents, and imaging technology have dramatically expanded the range of tools available for the study of the cytoskeleton. In Cytoskeleton Methods and Protocols, Ray Gavin brings together an international panel of experienced researchers to detail the readily reproducible methods that utilize biochemistry, immunology, genetics, microscopy, and image analysis for investigating cytoskeleton structure and function. Each protocol contains proven step-by-step instructions that enable both the novice and experienced researcher to achieve successful experimental results. The protocols utilize diverse model systems in a variety of organisms, including Saccharomyces, Micrasterias, Tetrahymena, Drosophila, Spisula, and Xenopus. Microscopy applications include digital-video microscopy and computer-assisted systems for the evaluation of cell motility and morphology. Help notes and tips accompany each protocol and provide additional, often unpublished, information that can make the difference between success and failure. State-of-the-art and highly practical, Cytoskeleton Methods and Protocols makes available a diverse collection of powerful experimental systems and tools for successfully studying cytoskeleton structure and function. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Cytology. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Cell Biology. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gavin, Ray H. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9780896037717 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781617371813 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781489942180 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 161 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1385/1592590519">https://doi.org/10.1385/1592590519</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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