GHU Logo
The Great Heights University Library
Academic discovery, digital access, and research support

Mycobacteria Protocols (Record no. 7340)

MARC details
000 -LEADER
fixed length control field 04548nam a22004695i 4500
001 - CONTROL NUMBER
control field 978-1-4939-2450-9
003 - CONTROL NUMBER IDENTIFIER
control field DE-He213
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20260610083702.0
007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION
fixed length control field cr nn 008mamaa
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 150316s2015 xxu| s |||| 0|eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 9781493924509
-- 978-1-4939-2450-9
024 7# - OTHER STANDARD IDENTIFIER
Standard number or code 10.1007/978-1-4939-2450-9
Source of number or code doi
050 #4 - LIBRARY OF CONGRESS CALL NUMBER
Classification number QR
072 #7 - SUBJECT CATEGORY CODE
Subject category code PSG
Source bicssc
072 #7 - SUBJECT CATEGORY CODE
Subject category code SCI045000
Source bisacsh
072 #7 - SUBJECT CATEGORY CODE
Subject category code PSG
Source thema
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 579
Edition information 23
245 10 - TITLE STATEMENT
Title Mycobacteria Protocols
Medium [electronic resource] /
Statement of responsibility, etc. edited by Tanya Parish, David M. Roberts.
250 ## - EDITION STATEMENT
Edition statement 3rd ed. 2015.
264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture New York, NY :
Name of producer, publisher, distributor, manufacturer Springer New York :
-- Imprint: Humana,
Date of production, publication, distribution, manufacture, or copyright notice 2015.
300 ## - online resource
Extent XII, 416 p. 88 illus., 37 illus. in color.
Other physical details online resource.
336 ## - CONTENT TYPE
Content type term text
Content type code txt
Source rdacontent
337 ## - MEDIA TYPE
Media type term computer
Media type code c
Source rdamedia
338 ## - CARRIER TYPE
Carrier type term online resource
Carrier type code cr
Source rdacarrier
347 ## - DIGITAL FILE CHARACTERISTICS
File type text file
Encoding format PDF
Source rda
490 1# - SERIES STATEMENT
Series statement Methods in Molecular Biology,
International Standard Serial Number 1940-6029 ;
Volume/sequential designation 1285
505 0# - FORMATTED CONTENTS NOTE
Formatted contents note Whole Genome Sequencing for Comparative Genomics and De Novo Genome Assembly -- Whole Transcriptome Sequencing for High-resolution Transcriptomic Analysis in Mycobacterium tuberculosis -- RNA Sequencing for Transcript 5’-end Mapping in Mycobacteria -- Fractionation and Analysis of Mycobacterial Proteins -- Lipid and Lipoarabinomannan Isolation and Characterization -- Metabolomics of Mycobacterium tuberculosis -- Electroporation of Mycobacteria -- Targeted Gene Knockout and Essentiality Testing by Homologous Recombination -- Construction of Conditional Knockdown Mutants in Mycobacteria -- Mycobacterial Recombineering -- In vitro Models that Utilize Hypoxia to Induce Non-replicating Persistence in Mycobacteria -- Genetic Dissection of Mycobacterial Biofilms -- Measuring Efflux and Permeability in Mycobacteria -- Single-cell Analysis of Mycobacteria using Microfluidics and Time-lapse Microscopy -- Antimicrobial Susceptibility Testing for Mycobacterium sp. -- Determination of Compound Kill Kinetics Against Mycobacterium tuberculosis -- Microplate Alamar Blue Assay (MABA) and Low Oxygen Recovery Assay (LORA) for Mycobacterium tuberculosis -- A Multi-Stress Model for High Throughput Screening Against Non-replicating Mycobacterium tuberculosis -- Isolation and Characterization of Compound-resistant Isolates of Mycobacterium tuberculosis -- Macrophage Infection Models for Mycobacterium tuberculosis -- Infection of Human Neutrophils to Study Virulence Properties of Mycobacteriumtuberculosis -- Isolation of Bead-phagosomes to Study Virulence Function of M. tuberculosis Cell Wall Lipids -- Live Imaging of Mycobacterium marinum Infection in Dictyostelium discoideum -- Testing Chemical and Genetic Modulators in Mycobacterium tuberculosis Infected Cells using Phenotypic Assays.  .
520 ## - SUMMARY, ETC.
Summary, etc. Updated and revised, this thorough volume provides a selection of the newest methods, as well as some of the basic methods required for a mycobacterial research laboratory.  Mycobacteria Protocols, Third Edition guides readers through fractionation and analysis of macromolecules, from nucleic acids to proteins, complex lipids, and metabolites.  Detailed and comprehensive protocols are provided for protein and lipid/glycolipid analysis using well-established methods; these are now complemented by a metabolomics chapter in which the complement of metabolites can be profiled. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Mycobacteria Protocols, Third Edition will be a resource both to those working in the field, and to newcomers.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Microbiology.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Microbiology.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Parish, Tanya.
Relator term editor.
Authority record control number or standard number (orcid)0000-0001-7507-0423
Real World Object URI https://orcid.org/0000-0001-7507-0423
Relationship edt
-- http://id.loc.gov/vocabulary/relators/edt
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Roberts, David M.
Relator term editor.
Relationship edt
-- http://id.loc.gov/vocabulary/relators/edt
710 2# - ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element SpringerLink (Online service)
773 0# - HOST ITEM ENTRY
Title Springer Nature eBook
776 08 - ADDITIONAL PHYSICAL FORM ENTRY
Relationship information Printed edition:
International Standard Book Number 9781493924493
776 08 - ADDITIONAL PHYSICAL FORM ENTRY
Relationship information Printed edition:
International Standard Book Number 9781493924516
776 08 - ADDITIONAL PHYSICAL FORM ENTRY
Relationship information Printed edition:
International Standard Book Number 9781493955824
830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE
Uniform title Methods in Molecular Biology,
International Standard Serial Number 1940-6029 ;
Volume/sequential designation 1285
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://doi.org/10.1007/978-1-4939-2450-9">https://doi.org/10.1007/978-1-4939-2450-9</a>
912 ## -
-- ZDB-2-PRO

No items available.