Oligonucleotide Synthesis (Record no. 7162)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04865nam a22004455i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-59259-823-6 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083659.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 100301s2005 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781592598236 |
| -- | 978-1-59259-823-6 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1385/1592598234 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | RS1-441 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | MQP |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | MED072000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | MQP |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 615.1 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Oligonucleotide Synthesis |
| Medium | [electronic resource] : |
| Remainder of title | Methods and Applications / |
| Statement of responsibility, etc. | edited by Piet Herdewijn. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 2005. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Totowa, NJ : |
| Name of producer, publisher, distributor, manufacturer | Humana Press : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2005. |
| 300 ## - online resource | |
| Extent | XVI, 436 p. 142 illus. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 288 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Synthesis of DNA Using a New Two-Step Cycle -- RNA Synthesis Using 2?-O-(Tert-Butyldimethylsilyl) Protection -- RNA Oligonucleotide Synthesis Via 5?-Silyl-2?-Orthoester Chemistry -- Dimethylthiarum Disulfide -- Assay for Evaluating Ribonuclease H-Mediated Degradation of RNA-Antisense Oligonucleotide Duplexes -- Di- and Oligonucleotide Synthesis Using H-Phosphonate Chemistry -- Solid-Phase Synthesis of Circular Oligonucleotides -- Locked Nucleic Acid Synthesis -- Synthesis of DNA Mimics Representing HypNA-pPNA Hetero-Oligomers -- Base-Modified Oligonucleotides With Increased Duplex Stability -- of Hypermodified Nucleotides in RNA -- Chemical Methods for Peptide-Oligonucleotide Conjugate Synthesis -- Biotin-Labeled Oligonucleotides With Extraordinarily Long Tethering Arms -- Universal Labeling Chemistry for Nucleic Acid Detection on DNA Chips -- Postsynthetic Functionalization of Triple Helix-Forming Oligonucleotides -- Fluorescence-Based On-Line Detection as an Analytical Tool in RNA Electrophoresis -- Design and Optimization of Molecular Beacon Real-Time Polymerase Chain Reaction Assays -- High-Throughput Production of Optimized Primers (Fimers) for Whole-Genome Direct Sequencing -- Nonenzymatic Template-Directed RNA Synthesis -- DNase I Footprinting of Small Molecule Binding Sites on DNA -- Gene Targeting Using Peptide Nucleic Acid -- Nucleic Acid Library Construction Using Synthetic DNA Constructs -- In Vitro Selection From Combinatorial Nucleic Acid Libraries -- In Vitro Selection Procedures for Identifying DNA and RNA Aptamers Targeted to Nucleic Acids and Proteins -- Short Double-Stranded Ribonucleic Acid as Inhibitor of Gene Expression by the Interference Mechanism. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Nucleic acids chemistry has progressed rapidly in the last 10 years with the introduction of many new techniques and the development of many novel therapeutic entities. In Oligonucleotide Synthesis: Methods and Applications, laboratory experts describe in step-by-step detail the powerful new techniques they have developed for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are the synthesis of phosphorothioates via new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, triple helix formation, and a novel two-step process that yields a high purity DNA that is less costly. Novel applications include the use of molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules to DNA, nucleic acid libraries, optimization of RNA synthesis for siRNA applications, and the use of small interference RNA (siRNA) as an inhibitor of gene expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Oligonucleotide Synthesis: Methods and Applications offers today's investigators not only insight into important new biotechnologies, but also a full range of the detailed protocols needed to work successfully in DNA synthesis and its applications today. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Pharmacy. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Pharmacy. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Herdewijn, Piet. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781588292339 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781617374418 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 288 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1385/1592598234">https://doi.org/10.1385/1592598234</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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