Molecular Toxicology Protocols (Record no. 6517)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 07068nam a22004815i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-59259-840-3 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083648.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 100301s2005 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781592598403 |
| -- | 978-1-59259-840-3 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1385/1592598404 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | RM300-666 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | MMG |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | MED071000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | MKG |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 615 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Molecular Toxicology Protocols |
| Medium | [electronic resource] / |
| Statement of responsibility, etc. | edited by Phouthone Keohavong, Stephen G. Grant. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 2005. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Totowa, NJ : |
| Name of producer, publisher, distributor, manufacturer | Humana Press : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2005. |
| 300 ## - online resource | |
| Extent | XIV, 489 p. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 291 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Analysis of DNA Adducts -- 32P-Postlabeling Analysis of DNA Adducts -- Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot -- DNA Isolation and Sample Preparation for Quantification of Adduct Levels by Accelerator Mass Spectrometry -- Fluoroimaging-Based Immunoassay of DNA Photoproducts in Ultraviolet-B-Irradiated Tadpoles -- Analysis of DNA Strand Cleavage at Abasic Sites -- Detection of Chromosomal and Genome-Wide Damage -- Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes for Chromosome Aberration Analysis Using Specific Whole-Chromosome DNA Hybridization Probes -- Mutagen-Induced Chromatid Breakage as a Marker of Cancer Risk -- Flow Cytometric Analysis of Micronuclei in Erythrocytes -- The Comet Assay -- Computerized Image Analysis Software for the Comet Assay -- The Comet-FISH Technique -- DNA Double-Strand Break Damage and Repair Assessed by Pulsed-Field Gel Electrophoresis -- Detection and Characterization of Surrogate Gene Mutation -- Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes -- Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems -- Methods for Detecting Somatic Mutations In Vitro -- Molecular Analysis of Mutations in the Human HPRT Gene -- Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes -- The GPA In Vivo Somatic Mutation Assay -- Flow Cytometric Measurement of Mutant T Cells With Altered Expression of TCR -- Detection and Characterization of Cancer Gene Mutation -- Mutation Screening of the TP53 Gene by Temporal Temperature Gradient Gel Electrophoresis -- Analysis of K-RAS and P53 Mutations in Sputum Samples -- Allele-Specific Competitive Blocker-PCR Detection of Rare Base Substitution -- Gel-Based Nonradioactive Single-Strand Conformational Polymorphism and Mutation Detection -- Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesions -- Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR -- Analysis of DNA Repair Mechanisms -- Microsatellite Instability -- Unscheduled DNA Synthesis -- Analysis of DNA Repair Using Transfection-Based Host Cell Reactivation -- An Immunoassay for Measuring Repair of Ultraviolet Photoproducts -- Analysis of DNA Double-Strand Break Repair by Nonhomologous End Joining in Cell-Free Extracts From Mammalian Cells -- Measuring Recombination Proficiency in Mouse Embryonic Stem Cells -- Array Technologies -- Strategies for Measurement of Biotransformation Enzyme Gene Expression -- Genotyping Technologies -- TaqMan® Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material -- Development of Quantitative Reverse Transcriptase PCR Assays for Measuring Gene Expression -- Apoptosis -- Quantification of Selective Phosphatidylserine Oxidation During Apoptosis -- Quantitative Method of Measuring Phosphatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance Spectroscopy and Annexin-Conjugated Iron -- Detection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation Assay. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | New state-of-the-art molecular techniques promise to transform the field of genetic toxicology by making it possible to directly detect genotoxic exposures and their consequences in humans, to identify the agent(s) involved, and to clinically manage the exposed population. In Molecular Toxicology Protocols, researchers from prominent universities and cancer centers around the world describe in detail their best techniques for analyzing genotoxic exposure and the resulting biological effects, including intermediate biomarkers such as DNA and chromosomal damage, mutations in reporter and oncogenes, and the earliest possible detection of cancer. The authors emphasize analytical methods specifically developed for use in human populations and in cancer patients, or in other in vivo systems such as transgenic mice. Among the applications detailed are the analysis of interactions of chemical and physical agents with cellular macromolecules, especially DNA, the assessment of medically relevant toxicity, and the individualized characterization of genetic damage and repair. There are also methods to assess and characterize the modulation of this damage and repair through individual differences in specific and genome-wide gene expression, including metabolic profiling and apoptotic capacity. These methods mark a shift in emphasis from studies of the agents themselves to the exposed population, and from studies of small populations with significant known exposures to a single agent, to studies of common diseases, such as breast cancer, caused by normal levels of generalized genotoxic exposure. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Molecular Toxicology Protocols offers agold-standard collection of cutting-edge techniques designed to investigate a broad range of exposures-endogenous, accidental, medical, environmental, and occupational-and their role in human carcinogenesis and other diseases of aging. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Pharmacology. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Biochemistry. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Pharmacology. |
| 650 24 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Biochemistry. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Keohavong, Phouthone. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Grant, Stephen G. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781588290847 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781617373473 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 291 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1385/1592598404">https://doi.org/10.1385/1592598404</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
No items available.