PCR (Record no. 6466)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 05269nam a22005775i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-0716-3358-8 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083647.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 230822s2023 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781071633588 |
| -- | 978-1-0716-3358-8 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1007/978-1-0716-3358-8 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | TP248.13-248.65 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | TCB |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI010000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | TCB |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.6 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | PCR |
| Medium | [electronic resource] : |
| Remainder of title | Methods and Protocols / |
| Statement of responsibility, etc. | edited by Lucília Domingues. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 2nd ed. 2023. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | New York, NY : |
| Name of producer, publisher, distributor, manufacturer | Springer US : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2023. |
| 300 ## - online resource | |
| Extent | XII, 254 p. 47 illus., 30 illus. in color. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Table of contents navigation |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Single logical reading order |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Short alternative textual descriptions |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Use of color is not sole means of conveying information |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Use of high contrast between text and background color |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Next / Previous structural navigation |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | All non-decorative content supports reading without sight |
| Source | onix |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 2967 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Digital PCR: A partitioning-based application for detection and surveillance of SARS-CoV-2 from sewage samples -- Digital PCR: A tool to authenticate herbal products and spice -- Emulsion Polymerase Chain Reaction coupled with Denaturant Gradient Gel Electrophoresis for microbial diversity studies -- Real-Time PCR High-Resolution Melting Assays for the Detection of Foodborne Pathogens -- High-Throughput real-time qPCR and High-Resolution Melting (HRM) assay for fungal detection in plant matrices -- Multiplex real-time PCR for the detection of Shiga Toxin-producing Escherichia coli in foods -- DNA isolation from cocoa-derived products and cocoa authentication by TaqMan real-time PCR -- Quantitative real-time PCR for the detection of allergenic species in foods -- Accurate absolute quantification of bacterial populations in mixed cultures by qPCR -- Real-time PCR method for assessment of ParA-mediated recombination efficiency in minicircle production -- Gene expression quantification from pathogenic bacterial biofilms by quantitative PCR -- A real-time quantitative PCR protocol for the quantification of plasmid copy number in Lactococcus lactis -- Improved PCR by the use of disruptors, a new class of oligonucleotide reagents -- Mitochondrial DNA D-loop amplification and sequencing for species differentiation in milk -- Long-range Polymerase Chain Reaction -- Megaprimer-based PCR to synthesise fusion genes for cloning -- Bacteria and Yeast ColonyPCR -- Inverse PCR for site-directed mutagenesis -- Optimized design of degenerate primers for PCR based on DNA or protein sequence comparisons. . |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. . |
| 532 8# - ACCESSIBILITY NOTE | |
| Summary of accessibility | Accessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com. |
| 532 8# - ACCESSIBILITY NOTE | |
| Summary of accessibility | No reading system accessibility options actively disabled |
| 532 8# - ACCESSIBILITY NOTE | |
| Summary of accessibility | Publisher contact for further accessibility information: accessibilitysupport@springernature.com |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Biotechnology. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Biotechnology. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Domingues, Lucília. |
| Relator term | editor. |
| Authority record control number or standard number | (orcid)0000-0003-1089-7627 |
| Real World Object URI | https://orcid.org/0000-0003-1089-7627 |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071633571 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071633595 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071633601 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 2967 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1007/978-1-0716-3358-8">https://doi.org/10.1007/978-1-0716-3358-8</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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