Schmidtea Mediterranea (Record no. 6462)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 05044nam a22006255i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-0716-3275-8 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083647.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 230710s2023 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781071632758 |
| -- | 978-1-0716-3275-8 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1007/978-1-0716-3275-8 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QH324 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PS |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PDN |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI008000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PS |
| Source | thema |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PDN |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 570.28 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Schmidtea Mediterranea |
| Medium | [electronic resource] : |
| Remainder of title | Methods and Protocols / |
| Statement of responsibility, etc. | edited by Luca Gentile. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 2023. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | New York, NY : |
| Name of producer, publisher, distributor, manufacturer | Springer US : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2023. |
| 300 ## - online resource | |
| Extent | XI, 280 p. 54 illus., 50 illus. in color. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Table of contents navigation |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Single logical reading order |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Short alternative textual descriptions |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Use of color is not sole means of conveying information |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Use of high contrast between text and background color |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Next / Previous structural navigation |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | All non-decorative content supports reading without sight |
| Source | onix |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 2680 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Building Phylogenies from Transcriptomic Data -- Isolation and Library Preparation of Planarian piRNAs -- Genome-Wide Analysis of Planarian piRNAs -- Combining Fluorescent In Situ Hybridization with Immunofluorescence and Lectin Staining in Planarians -- Colorimetric Whole-Mount In Situ Hybridization in Planarians -- Single Molecule Fluorescent In Situ Hybridization (smFISH) on Whole-Mount Planarians -- Whole-Mount In Situ Hybridization in Large Sexual Schmidtea mediterranea -- Preparing Planarian Cells for High-Content Fluorescence Microscopy Using RNA In Situ Hybridization and Immunocytochemistry -- An RNA/DNA-Based Flow Cytometry Approach for Isolating Slow-Cycling Stem Cells -- ACME Dissociation-Fixation, Flow Cytometry, and Cell Sorting of Freshwater Planarian Cells -- Papain-Based Dissociation of Schmidtea mediterranea Cells -- Live Immunostaining and Flow Cytometry of Schmidtea mediterranea Cells -- Live Imaging in Planarians: Immobilization and Real-Time Visualization of Reactive Oxygen Species -- A Planarian Model System to Study Host-Pathogen Interactions -- TUNEL Staining in Sections of Paraffin-Enabled Planarians -- Quantitative Analysis of Planarian Pigmentation -- mRNA Transfection of S. mediterranea Cells for Luminescence Analysis. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This detailed volume examines fine-tuned methodologies using the planarian species, Schmidtea mediterranea. The book features experimental protocols covering topics from in situ hybridization, immunohistochemistry, cell dissociation and flow cytometry, to pipelines for the analysis of large datasets, as in genomics and transcriptomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Schmidtea mediterranea: Methods and Protocols provides both experts in the field and newcomers with the best possible toolbox for their everyday lab work utilizing this valuable model. |
| 532 8# - ACCESSIBILITY NOTE | |
| Summary of accessibility | Accessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com. |
| 532 8# - ACCESSIBILITY NOTE | |
| Summary of accessibility | No reading system accessibility options actively disabled |
| 532 8# - ACCESSIBILITY NOTE | |
| Summary of accessibility | Publisher contact for further accessibility information: accessibilitysupport@springernature.com |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Biology |
| General subdivision | Technique. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Cytology. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Experimental Organisms. |
| 650 24 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Cell Biology. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gentile, Luca. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071632741 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071632765 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071632772 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 2680 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1007/978-1-0716-3275-8">https://doi.org/10.1007/978-1-0716-3275-8</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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