Epiblast Stem Cells (Record no. 6455)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04438nam a22004935i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-0716-2281-0 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083647.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 220429s2022 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781071622810 |
| -- | 978-1-0716-2281-0 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1007/978-1-0716-2281-0 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QH588.S83 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSC |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI072000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSC |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 571.6 |
| Edition information | 23 |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 616.02774 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Epiblast Stem Cells |
| Medium | [electronic resource] : |
| Remainder of title | Methods and Protocols / |
| Statement of responsibility, etc. | edited by Pierre Osteil. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 2022. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | New York, NY : |
| Name of producer, publisher, distributor, manufacturer | Springer US : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2022. |
| 300 ## - online resource | |
| Extent | XI, 303 p. 71 illus., 62 illus. in color. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 2490 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Establishment of Mouse Epiblast Stem Cells -- L-Proline Supplementation Drives Self-Renewing Mouse Embryonic Stem Cells to a Partially Primed Pluripotent State: The Early Primitive Ectoderm-Like Cell -- Generation of Epiblast-Like Cells -- Identification and Visualization of Protein Expression in Whole Mouse Embryos by Immunofluorescence -- Small Interfering RNA (siRNA) Transfection in Epiblast Stem Cells -- Measuring Endocytosis and Endosomal Uptake at Single Cell Resolution -- 3D Immunofluorescent Image Colocalization Quantification in Mouse Epiblast Stem Cells -- Flow Cytometric Characterization of Pluripotent Cell Protein Markers in Naïve, Formative, and Primed Pluripotent Stem Cells -- Exploring Chromatin Accessibility in Mouse Epiblast Stem Cells with ATAC-Seq -- A Reproducible and Dynamic Workflow for Analysis and Annotation of scRNA-Seq Data -- Complete Transcriptome Analysis by 5’-End Single-Cell RNA-Seq with Random Priming -- Global Proteomic Profiling of Embryonic Stem Cells Using iTRAQ Isobaric Tags with LC-MS/MS Quantification -- Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-Like Cells -- Generation of Gastruloids from Epiblast-Like Cells -- Definitive Endoderm from EpiSC Aggregates in Matrigel -- In Vitro Differentiation of Murine Embryonic Stem Cells (ESCs) into Primordial Germ Cell-Like Cells (PGCLCs) -- Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) into Human Primordial Germ Cell-Like Cells (hPGCLCs) In Vitro -- Differentiation of EpiLCs on Micro-Patterned Substrates Generated by Micro-Contact Printing -- Grafting of Epiblast Stem Cell into the Epiblast and Whole-Embryo Imaging to Unveil Lineage Competence -- Modeling Epiblast Shape in Implanting Mammalian Embryos. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This book aims to deliver the most up-to-date protocols in using epiblast stem cells (EpiSC) to answer critical questions on mammalian development. Beginning with a section on the spectrum of mouse primed pluripotent stem cells, the volume continues with chapters on toolkits to characterize and study primed stem cells, as well as primed stem cells to engineer gastrulation models. Written for the highly successful Method in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epiblast Stem Cells: Methods and Protocols serves as an ideal guide to overcome the reproducibility problem by gathering detailed state-of-the-art protocols to perform comprehensive analyses, enabling the reader to opt for the appropriate cell type to model the epiblast. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Stem cells. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Developmental biology. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Stem Cell Biology. |
| 650 24 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Developmental Biology and Stem Cells. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Osteil, Pierre. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071622803 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071622827 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071622834 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 2490 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1007/978-1-0716-2281-0">https://doi.org/10.1007/978-1-0716-2281-0</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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