TET Proteins and DNA Demethylation (Record no. 6119)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04681nam a22004935i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-0716-1294-1 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083641.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210519s2021 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781071612941 |
| -- | 978-1-0716-1294-1 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1007/978-1-0716-1294-1 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QD431-431.7 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSBC |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI049000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSB |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572.6 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | TET Proteins and DNA Demethylation |
| Medium | [electronic resource] : |
| Remainder of title | Methods and Protocols / |
| Statement of responsibility, etc. | edited by Ozren Bogdanovic, Michiel Vermeulen. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 2021. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | New York, NY : |
| Name of producer, publisher, distributor, manufacturer | Springer US : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2021. |
| 300 ## - online resource | |
| Extent | X, 320 p. 56 illus., 46 illus. in color. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 2272 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Reduced Bisulfite-Sequencing: Quantitative Base-Resolution Sequencing of 5-Formylcytosine -- Aba-Seq: High-Resolution Enzymatic Mapping of Genomic 5-Hydroxymethylcytosine -- Estimating Global Methylation and Erasure Using Low-Coverage Whole Genome Bisulfite Sequencing (WGBS) -- ELISA-Based Quantitation of Global 5hmC Levels -- Avidin-Biotin ELISA-Based Detection of 5hmC -- Quantification of DNA Methylation and Its Oxidized Derivatives using LC-MS -- Distinguishing Active Versus Passive DNA Demethylation using Illumina MethylationEPIC BeadChip Microarrays -- Bioinformatic Estimation of DNA Methylation and Hydroxymethylation Proportions -- TAB-seq and ACE-seq Data Processing for Genome-Wide DNA Hydroxymethylation Profiling -- Genomic Targeting of TET Activity for Targeted Demethylation using CRISPR/Cas9 -- High-Fidelity CRISPR/Cas9-Based Gene-Specific Hydroxymethylation -- Identifying Protein–(hydroxy)methylated DNA Interactions using Quantitative Interaction Proteomics -- Purification of TET Proteins -- Uncovering Sequence Specific Transcription Factors Interacting with TET2 -- ChIP-Sequencing of TET Proteins -- Harnessing Alternative Substrates to Probe TET Family Enzymes -- Generation and Molecular Characterization of Transient tet1/2/3 Zebrafish Knockouts. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This volume explores the latest methods used to study various aspects of TET proteins and their biology. Chapters in this book are divided into five parts. Part One describes technologies aimed at detecting and quantifying DNA methylation turnover using massively parallel sequencing, ELISA, and mass spectrometry approaches. Part Two looks at data analyses protocols for distinguishing acting versus passive DNA demethylation and estimation of 5mC and 5hmC levels. Part Three deals with a new topic that takes advantage of modified CRISPR/Cas9 genome editing systems to target DNA demethylation activity to genomic loci of interest. Part Four discusses protocols that detail how to purify TET proteins and unravel their protein interactions, and Part Five looks at the assessment of TET protein function and activity in vivo and in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, TET Proteins and DNA Demethylation: Methods and Protocols is a valuable resource that aims to help research scientists at all levels working in the fields of DNA demethylation dynamics. Chapters 3, 7 and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Proteins . |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Medical genetics. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Protein Biochemistry. |
| 650 24 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Medical Genetics. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bogdanovic, Ozren. |
| Relator term | editor. |
| Authority record control number or standard number | (orcid)0000-0001-5680-0056 |
| Real World Object URI | https://orcid.org/0000-0001-5680-0056 |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Vermeulen, Michiel. |
| Relator term | editor. |
| Authority record control number or standard number | (orcid)0000-0003-0836-6894 |
| Real World Object URI | https://orcid.org/0000-0003-0836-6894 |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071612934 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071612958 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071612965 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 2272 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1007/978-1-0716-1294-1">https://doi.org/10.1007/978-1-0716-1294-1</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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