DNA Manipulation and Analysis (Record no. 6066)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04933nam a22005775i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-0716-3004-4 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083640.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 230228s2023 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781071630044 |
| -- | 978-1-0716-3004-4 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1007/978-1-0716-3004-4 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QH426-470 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSAK |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI029000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSAK |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 576.5 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | DNA Manipulation and Analysis |
| Medium | [electronic resource] / |
| Statement of responsibility, etc. | edited by Garry Scarlett. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 2023. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | New York, NY : |
| Name of producer, publisher, distributor, manufacturer | Springer US : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2023. |
| 300 ## - online resource | |
| Extent | X, 249 p. 49 illus., 44 illus. in color. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Table of contents navigation |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Single logical reading order |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Short alternative textual descriptions |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Use of color is not sole means of conveying information |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Use of high contrast between text and background color |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | Next / Previous structural navigation |
| Source | onix |
| 341 0# - ACCESSIBILITY CONTENT | |
| Textual assistive features | All non-decorative content supports reading without sight |
| Source | onix |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 2633 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Classical Recombinant DNA Cloning -- A Sequence and Ligation-Independent Cloning (SLIC) Procedure for the Insertion of Genes into a Plasmid Vector -- Molecular cloning using in vivo DNA assembly -- Assembling multiple fragments: The Gibson Assembly -- TA cloning approaches to cloning DNA with damaged ends DNA -- PCR-based Assembly of Gene Sequences by Thermodynamically Balanced Inside-Out (TBIO) Gene Synthesis -- Random Mutagenesis by PCR -- In vitro Site Directed Mutagenesis -- Xenopus transgenesis using the pGateway system -- CRISPR/Cas9 gene disruption studies in F0 Xenopus tadpoles: understanding development and disease in the frog -- A CRISPR/Cas based method for precise DNA integration in Xenopus laevis oocytes followed by intracytoplasmic sperm injection (ICSI) fertilisation -- A Lambda-Exonuclease SELEX Method for Generating Aptamers to Bacterial Targets. -Generation of functional-RNA arrays by in vitrotranscription and in situ RNA capture for the detection of RNA-RNA interactions -- Chemical synthesis of oligonucelotide sequences: Phosphoramidite chemistry -- Low Throughput Direct Cycle Sequencing of Polymerase Chain Reaction (PCR) Products -- Next generation Sequencing for mixed samples -- Ethics, Legality and Safety for Geneticists. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This volume details technologies on recombinant DNA and nucleic acid manipulation that underpin much of the biological sciences and instructions on how to conduct them successfully. Chapters guide the reader through the basics of oligonucleotide synthesis and DNA sequencing; recombinant DNA plasmid work; cell-free experiments and the latest developments in CRISPR approaches to genome modification. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, DNA Manipulation and Analysis aims to be the comprehensive guide for life scientists moving into the field of recombinant DNA and nucleic acid manipulation. |
| 532 8# - ACCESSIBILITY NOTE | |
| Summary of accessibility | Accessibility summary: This PDF does not fully comply with PDF/UA standards, but does feature limited screen reader support, described non-text content (images, graphs), bookmarks for easy navigation and searchable, selectable text. Users of assistive technologies may experience difficulty navigating or interpreting content in this document. We recognize the importance of accessibility, and we welcome queries about accessibility for any of our products. If you have a question or an access need, please get in touch with us at accessibilitysupport@springernature.com. |
| 532 8# - ACCESSIBILITY NOTE | |
| Summary of accessibility | No reading system accessibility options actively disabled |
| 532 8# - ACCESSIBILITY NOTE | |
| Summary of accessibility | Publisher contact for further accessibility information: accessibilitysupport@springernature.com |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Genetics. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Genetics and Genomics. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Scarlett, Garry. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071630037 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071630051 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071630068 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 2633 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1007/978-1-0716-3004-4">https://doi.org/10.1007/978-1-0716-3004-4</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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