Viroids (Record no. 5920)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 04757nam a22005055i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-0716-1464-8 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083637.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 211130s2022 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781071614648 |
| -- | 978-1-0716-1464-8 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1007/978-1-0716-1464-8 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QK981-981.7 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PST |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSAK |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI011000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSTB |
| Source | thema |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSAK |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 581.35 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Viroids |
| Medium | [electronic resource] : |
| Remainder of title | Methods and Protocols / |
| Statement of responsibility, etc. | edited by Ayala L. N. Rao, Irene Lavagi-Craddock, Georgios Vidalakis. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 2022. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | New York, NY : |
| Name of producer, publisher, distributor, manufacturer | Springer US : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2022. |
| 300 ## - online resource | |
| Extent | XIII, 375 p. 81 illus., 48 illus. in color. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 2316 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Study and Detection of Citrus Viroids in Woody Hosts -- Detection and Characterization of Viroids via Biological Assays on Herbaceous Hosts -- Analysis on RNA Motif-Based RNA Trafficking in Plants -- Isolation and Transfection of Citrus Protoplasts with Citrus Exocortis -- High-Throughput RNA Extraction from Citrus Tissues for the Detection of Viroids -- Extraction and Purification of Viroids from Herbaceous Hosts -- Detection of Viroids by sPAGE Gel Electrophoresis -- Two-Dimensional Polyacrylamide-Gel Electrophoresis Analysis of Viroid RNAs -- A Simplified Dot-Blot Hybridization Protocol for Potato spindle tuber viroid Detection in Solanaceae -- Gel Blot Hybridization for Viroids -- Tissue Print Hybridization for Detection and Characterization of Viroids -- Fluorescein-Based Electrophoretic Mobility Shift Assay -- Detection of Viroids by RT-PCR -- Detection of Viroids using RT-qPCR -- Multiplex RT-PCR -- Real-TimeDetection of Viroids using Singleplex and Multiplex Quantitative Polymerase Chain Reaction -- Reverse Transcription-Loop Mediated Isothermal AMPlification (RT-LAMP) at the Service of Viroid Detection -- SYBR ® Green RT-qPCR for the Universal Detection of Citrus Viroids -- Detection of Avocado sunblotch and Other Viroids using RNA Filter Paper Capture and RT-PCR -- Cloning and Sequencing of Viroids -- QuantiGene Plex Assay: A Method for High-Throughput Multiplex Citrus Viroid Detection and Identification -- Culture-Independent Discovery of Viroids by Deep Sequencing and Computational Algorithms -- An In Silico Detection of a Citrus Viroid from Raw High Throughput Sequencing Data -- Detection of Viroid RNA and vd-siRNA in N. benthamiana Plants: Northern Blot Analyses for Viroid and vd-siRNAs -- Production of Recombinant RNA in Escherichia coli Using Eggplant Latent Viroid as a Scaffold -- Predicting the Structure of a Viroid. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This volume explores the latest methods used by researchers to study the detection, characterization, and various aspects of viroids. The chapters in this book are organized into seven parts and cover topics such as detection methods based on the biology of viroids; detection techniques based on electrophoresis and hybridization techniques; PCR-based techniques that provide high degrees of sensitivity; emerging area of nucleic acid sequence-based technology; and emerging techniques in viroid research such as RNA silencing, splicing, and viroid structure. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Viroids: Methods and Protocols is a valuable resource for researchers and graduate students who are working with viroid diseases. . |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Plant genetics. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Plant Genetics. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rao, Ayala L. N. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lavagi-Craddock, Irene. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Vidalakis, Georgios. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071614631 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071614655 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781071614662 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 2316 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1007/978-1-0716-1464-8">https://doi.org/10.1007/978-1-0716-1464-8</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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