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Proteomic Profiling (Record no. 5822)

MARC details
000 -LEADER
fixed length control field 06538nam a22004815i 4500
001 - CONTROL NUMBER
control field 978-1-0716-1186-9
003 - CONTROL NUMBER IDENTIFIER
control field DE-He213
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20260610083635.0
007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION
fixed length control field cr nn 008mamaa
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 210109s2021 xxu| s |||| 0|eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 9781071611869
-- 978-1-0716-1186-9
024 7# - OTHER STANDARD IDENTIFIER
Standard number or code 10.1007/978-1-0716-1186-9
Source of number or code doi
050 #4 - LIBRARY OF CONGRESS CALL NUMBER
Classification number QD431-431.7
072 #7 - SUBJECT CATEGORY CODE
Subject category code PSBC
Source bicssc
072 #7 - SUBJECT CATEGORY CODE
Subject category code SCI049000
Source bisacsh
072 #7 - SUBJECT CATEGORY CODE
Subject category code PSB
Source thema
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 572.6
Edition information 23
245 10 - TITLE STATEMENT
Title Proteomic Profiling
Medium [electronic resource] :
Remainder of title Methods and Protocols /
Statement of responsibility, etc. edited by Anton Posch.
250 ## - EDITION STATEMENT
Edition statement 2nd ed. 2021.
264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture New York, NY :
Name of producer, publisher, distributor, manufacturer Springer US :
-- Imprint: Humana,
Date of production, publication, distribution, manufacture, or copyright notice 2021.
300 ## - online resource
Extent XV, 592 p. 131 illus., 91 illus. in color.
Other physical details online resource.
336 ## - CONTENT TYPE
Content type term text
Content type code txt
Source rdacontent
337 ## - MEDIA TYPE
Media type term computer
Media type code c
Source rdamedia
338 ## - CARRIER TYPE
Carrier type term online resource
Carrier type code cr
Source rdacarrier
347 ## - DIGITAL FILE CHARACTERISTICS
File type text file
Encoding format PDF
Source rda
490 1# - SERIES STATEMENT
Series statement Methods in Molecular Biology,
International Standard Serial Number 1940-6029 ;
Volume/sequential designation 2261
505 0# - FORMATTED CONTENTS NOTE
Formatted contents note How Modern Mass Spectrometry Can Solve Ancient Questions: A multi-Omics Study of the Stomach Content of the Oldest Human Ice Mummy, the 5300-Year-Old Iceman or Oetzi -- Step-by-Step Sample Preparation of Proteins for Mass Spectrometric Analysis -- Lab-on-a-Filter Techniques for Economical, Effective, and Flexible Proteome Analysis -- Released N-Glycan Analysis for Biotherapeutic Development Using Liquid Chromatography and Mass Spectrometry -- Comprehensive Protocol to Simultaneously Study Protein Phosphorylation, Acetylation, and N-Linked Sialylated Glycosylation -- Phos-Tag Fluorescent Gel Staining for the Quantitative Detection of His- and Asp-Phosphorylated Proteins -- Polyubiquitin Profile in Down Syndrome and Alzheimer Disease Brain -- Platform Methods to Characterize the Charge Heterogeneity of Three Common Protein Therapeutics by Imaged Capillary Isoelectric Focusing -- A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes -- Human Plasma Extracellular Vesicle Isolation and Proteomic Characterization for the Optimization of Liquid Biopsy in Multiple Myeloma -- Isolation of Extracellular Vesicles for Proteomic Profiling -- Isolation of Proteins from Extracellular Vesicles (EVs) for Mass Spectrometry-Based Proteomic Analyses -- Flow Cytometry as an Important Tool in Proteomic Profiling -- Improved Immunoprecipitation to Mass Spectrometry Method for Enrichment of Low-Abundant Protein Targets -- Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors -- Bead-Based Multiplex Immunoassays: Procedures, Tips, and Tricks -- Immunoaffinity-Based Liquid Chromatography Mass Spectrometric Assay to Accurately Quantify the Protein Concentration of HMGB1 in EDTA Plasma -- Recombinant Anti-Idiotypic Antibodies in Ligand Binding Assays for Antibody Drug Development -- cDNA Display-Mediated Immuno-PCR (cD-IPCR): An Ultrasensitive Immunoassay for Biomolecular Detection -- Chromatin Immunoprecipitation (ChIP) to Study DNA-Protein Interactions -- Profiling Protein-DNA Interactions by Chromatin Immunoprecipitation in Arabidopsis -- Biotin Proximity Labeling for Protein-Protein Interaction Discovery: The BioID Method -- Studying OTUD6B-OTUB1 Protein-Protein Interaction by Low-Throughput GFP-Trap Assays and High-Throughput AlphaScreen Assays -- Thermal Shift Assay for Exploring Interactions between Fatty Acid-Binding Protein and Inhibitors -- Isolation and Purification of Mitochondria from Cell Culture for Proteomic Analyses -- Investigating the Adipose Tissue Secretome: A Protocol to Generate High Quality Samples Appropriate for Comprehensive Proteomic Profiling -- Methods for Proteomics-Based Analysis of the Human Muscle Secretome Using an In Vitro Exercise Model -- Western Blotting Using In-Gel Protein Labeling as a Normalization Control: Advantages of Stain-Free Technology -- Technical Considerations for Contemporary Western Blot Techniques -- Simple Western: Bringing the Western Blot into the 21st Century -- Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests -- Sample Preparation of Secreted Mammalian Host Cell Proteins and their Characterization by Two-Dimensional Electrophoresis and Western Blotting -- Quantitative Proteomic Analysis Using Formalin-Fixed Paraffin-Embedded (FFPE) Human Cardiac Tissue -- Chloroplast Isolation and Enrichment of Low Abundant Proteins by Affinity Chromatography for Identification in Complex Proteomes -- Principles of Protein Labeling Techniques -- Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization.
520 ## - SUMMARY, ETC.
Summary, etc. This detailed new edition presents the latest developments of the main pillars of protein analysis, namely sample preparation, separation, and characterization. Core areas in this volume are protocols for the analysis of post-translational modifications and protein interaction partners, followed by sophisticated procedures to enrich for extracellular vesicles and organelles, along with several types of protein immuno-assays complemented by various methods for the characterization of antibodies and host-cell protein analysis. Last but not least, a few standard sample preparation protocols and recent advances concerning immuno-chemical detection of proteins are included as well. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Proteomic Profiling: Methods and Protocols, Second Edition serves as an ideal reference for students of biochemistry, biomedicine, biology, and genomics and will be an invaluable source for the experienced, practicing scientist as well.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Proteins .
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Cytology.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Protein Biochemistry.
650 24 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Cell Biology.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Posch, Anton.
Relator term editor.
Relationship edt
-- http://id.loc.gov/vocabulary/relators/edt
710 2# - ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element SpringerLink (Online service)
773 0# - HOST ITEM ENTRY
Title Springer Nature eBook
776 08 - ADDITIONAL PHYSICAL FORM ENTRY
Relationship information Printed edition:
International Standard Book Number 9781071611852
776 08 - ADDITIONAL PHYSICAL FORM ENTRY
Relationship information Printed edition:
International Standard Book Number 9781071611876
776 08 - ADDITIONAL PHYSICAL FORM ENTRY
Relationship information Printed edition:
International Standard Book Number 9781071611883
830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE
Uniform title Methods in Molecular Biology,
International Standard Serial Number 1940-6029 ;
Volume/sequential designation 2261
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://doi.org/10.1007/978-1-0716-1186-9">https://doi.org/10.1007/978-1-0716-1186-9</a>
912 ## -
-- ZDB-2-PRO

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