Protein Targeting Protocols (Record no. 5380)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03903nam a22004575i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-1-59259-572-3 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260610083628.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 100301s1998 xxu| s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781592595723 |
| -- | 978-1-59259-572-3 |
| 024 7# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | 10.1385/0896034879 |
| Source of number or code | doi |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QD415-436 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSB |
| Source | bicssc |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI007000 |
| Source | bisacsh |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | PSB |
| Source | thema |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572 |
| Edition information | 23 |
| 245 10 - TITLE STATEMENT | |
| Title | Protein Targeting Protocols |
| Medium | [electronic resource] / |
| Statement of responsibility, etc. | edited by Roger A. Clegg. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 1998. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Totowa, NJ : |
| Name of producer, publisher, distributor, manufacturer | Humana Press : |
| -- | Imprint: Humana, |
| Date of production, publication, distribution, manufacture, or copyright notice | 1998. |
| 300 ## - online resource | |
| Extent | XII, 331 p. |
| Other physical details | online resource. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 347 ## - DIGITAL FILE CHARACTERISTICS | |
| File type | text file |
| Encoding format | |
| Source | rda |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 88 |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Surface Plasmon Resonance -- Microcalorimetry of Protein-Protein Interactions -- Partitioning of Proteins in Triton X-114 -- Co-Immunoprecipitation -- Overlay, Ligand Blotting, and Band-Shift Techniques to Study Kinase Anchoring -- Size-Exclusion Chromatography -- Overlay and Bead Assay -- Use of GST-Fusion and Related Constructs for the Identification of Interacting Proteins -- Molecular Genetic Approaches I -- Molecular Genetic Approaches II -- Molecular Genetic Approaches III -- Molecular Genetic Approaches IV -- Use of Synthetic Peptides in the Dissection of Protein-Targeting Interactions -- SH2 and SH3 Domains -- PH Domain of Serine-Threonine Protein Kinase B (RAC-PKB) -- Membrane Targeting via Protein N-Myristoylation -- Membrane Targeting via Protein Palmitoylation -- Agonist-Mediated Turnover of G-Protein ?-Subunit Palmitoyl Groups -- Membrane Targeting via Protein Prenylation -- Nuclear Protein Import in a Permeabilized Cell Assay -- Clathrin-Coated Vesicles and Targeting -- Analysis of the Sorting of Secretory Proteins to the Regulated Secretory Pathway. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | In Protein Targeting Protocols, master experimentalists describe, largely from the perspective of intracellular signaling, their best time-tested methods for detecting and analyzing the proteins and protein motifs involved in cellular targeting interactions. The techniques range from basic methods that reveal the existence of targeting interactions and characterize physicochemical properties to those for analyzing the more structurally elaborate systems involved in the movement of molecules between cellular compartments, with examples taken from nuclear transport, clathrin-coated vesicle traffic, and secretory sorting. The themes of targeting domains, particularly the SH-2, SH-3, and PH domains, and the principal targeting of lipid modifications-myristoylation, reversible palmitoylation, prenylation-are fully explored. Protein Targeting Protocols offers a valuable collection of both basic and state-of-the-art techniques suitable for use in all areas of molecular cell biology by researchers interested in defining and characterizing the wide variety of protein interactions underlying targeting mechanisms. Readers will find it serves well as a firm methodological foundation for laboratories newly entering this field and as an indispensable reference work for those already well-established in it. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Biochemistry. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Biochemistry. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Clegg, Roger A. |
| Relator term | editor. |
| Relationship | edt |
| -- | http://id.loc.gov/vocabulary/relators/edt |
| 710 2# - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY | |
| Title | Springer Nature eBook |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9780896034501 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9780896034877 |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Printed edition: |
| International Standard Book Number | 9781489942999 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in Molecular Biology, |
| International Standard Serial Number | 1940-6029 ; |
| Volume/sequential designation | 88 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://doi.org/10.1385/0896034879">https://doi.org/10.1385/0896034879</a> |
| 912 ## - | |
| -- | ZDB-2-PRO |
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