Chromaffin Cells [electronic resource] : Methods and Protocols /
Chromaffin Cells Methods and Protocols / [electronic resource] :
edited by Ricardo Borges.
- 1st ed. 2023.
- XIV, 373 p. 89 illus., 68 illus. in color. online resource.
- Methods in Molecular Biology, 2565 1940-6029 ; .
- Methods in Molecular Biology, 2565 .
Rat pheochromocytoma PC12 cells in culture -- Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantation -- Immunocytochemistry of acutely isolated adrenal medullary chromaffin cells -- Transmission electron microscopy: a method for studying the adrenochromaffin cells -- Immunogold for protein location in chromaffin cells -- Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopy -- Online detection of catecholamine release from the perfused rat adrenal gland -- On-line recording of perifused chromaffin cells -- Recording of chromaffin cell electrical activity in situ in acute adrenal slices -- Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive Rats -- Measurements of calcium in chromaffin cell organelles using targeted aequorins -- Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurements -- Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cell -- Quantal release analysis of electrochemically active molecules using single-cell amperometry -- Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAs -- Vesicle collision protocols for the study of quantum size and exocytotic fraction released -- Patch Amperometry and Intracellular Patch Electrochemistry -- Artificial cells for dissecting exocytosis -- Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cells -- Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells -- Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging -- Determinationof catecholamines in a small volume (25µl) of plasma from conscious mouse tail vein -- Quantification of chromogranin A and its fragments in biological fluids -- Cytotoxicity models in chromaffin cells to evaluate neuroprotective compounds.
This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. .
9781071626719
10.1007/978-1-0716-2671-9 doi
Cytology.
Pharmacology.
Cell Biology.
Pharmacology.
QH573-671
571.6
Rat pheochromocytoma PC12 cells in culture -- Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantation -- Immunocytochemistry of acutely isolated adrenal medullary chromaffin cells -- Transmission electron microscopy: a method for studying the adrenochromaffin cells -- Immunogold for protein location in chromaffin cells -- Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopy -- Online detection of catecholamine release from the perfused rat adrenal gland -- On-line recording of perifused chromaffin cells -- Recording of chromaffin cell electrical activity in situ in acute adrenal slices -- Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive Rats -- Measurements of calcium in chromaffin cell organelles using targeted aequorins -- Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurements -- Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cell -- Quantal release analysis of electrochemically active molecules using single-cell amperometry -- Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAs -- Vesicle collision protocols for the study of quantum size and exocytotic fraction released -- Patch Amperometry and Intracellular Patch Electrochemistry -- Artificial cells for dissecting exocytosis -- Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cells -- Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells -- Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging -- Determinationof catecholamines in a small volume (25µl) of plasma from conscious mouse tail vein -- Quantification of chromogranin A and its fragments in biological fluids -- Cytotoxicity models in chromaffin cells to evaluate neuroprotective compounds.
This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. .
9781071626719
10.1007/978-1-0716-2671-9 doi
Cytology.
Pharmacology.
Cell Biology.
Pharmacology.
QH573-671
571.6