Plant Immunity [electronic resource] : Methods and Protocols /
Plant Immunity Methods and Protocols / [electronic resource] :
edited by Tsutomu Kawasaki, Hirofumi Yoshioka.
- 1st ed. 2026.
- XI, 266 p. 153 illus., 122 illus. in color. online resource.
- Methods in Molecular Biology, 3012 1940-6029 ; .
- Methods in Molecular Biology, 3012 .
Lumi-Map, a Luciferase Bioluminescence Screen of Mutants Combined with MutMap Allows Rapid Dissection of Plant Immune Signaling Pathways -- Identification of Phosphorylation and Protein Interactions of Plant Pattern Recognition Receptors Through Immunoprecipitation -- Plant-Based Ligand-Depletion Binding Assay for Assessing Pattern Recognition Receptor-Ligand Interactions -- Plate Reader-Based Quantification of Cellular Calcium Dynamics in Immune Responses Using the Genetically-Encoded Calcium Indicator GCaMP -- Visualization of Calcium Decoding via a FRET-Based Reporter of Calcium-Decoder Conformation -- Visualization and Quantification of Apoplastic ROS by Plant Immune NADPH Oxidase Utilizing Chemiluminescence -- Detection of Phosphorylated NADPH Oxidase by Antibody During Plant Immune Responses -- The Application of RenSeq for Pan-NLRome Analysis -- Blue Native-PAGE Assay of NLR Protein Complexes Combined with Genetic Complementation in Nicotiana benthamiana -- Quantification of NLR-Induced Cell Death Using Rice Protoplasts -- Split-TurboID-Based Proximity Labeling for Probing the Composition of Spatiotemporally Defined Protein Complexes In Planta -- Virus-Induced Gene Silencing for Identifying Autoimmune Regulators in Arabidopsis -- Bioinformatics Workflow for Co-Transcriptome Analysis of Plant-Bacterial Interactions -- Nuclei Isolation from Mature Leaves of Arabidopsis thaliana Infected by Bacterial Pathogens for Single-Nucleus Multiomics -- Multimodal Analysis of Phytoalexin Synthesis in Arabidopsis by Mass Spectrometry Imaging and Fluorescent Microscopy -- Bioluminescence-Based Growth Quantification of the Phytopathogenic Bacterium Pseudomonas syringae pv. tomato DC3000 -- Genome Editing for Developing Disease-Resistant Plants.
This volume explores plant immunity through protocols for recently established genetic and biochemical techniques to elucidate immune mechanisms mediated by pattern-recognition receptors (PRRs) and nucleotide-binding leucine-rich repeat receptors (NLRs). The book covers the identification of PRRs, their associated downstream immune regulators, PRR-ligand interaction-based immune responses, identification of NLR complex components, pan-NLRome analysis, resistosome assays, and cell death evaluation. Additionally, it presents protocols for co-transcriptome analysis of plant-pathogen interactions, single-nucleus multi-omics, metabolite imaging, and bioluminescence-based quantification of pathogen growth, as well as genome editing tools that contribute to the development of disease-resistant plants. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Immunity: Methods and Protocols serves as an ideal guide for researchers aiming to accelerate ongoing research and advances in the field of plant immunity.
9781071651384
10.1007/978-1-0716-5138-4 doi
Plants--Disease and pest resistance.
Botany.
Plant Immunity.
Plant Science.
SB750 QR185.2-.6
571.9642
Lumi-Map, a Luciferase Bioluminescence Screen of Mutants Combined with MutMap Allows Rapid Dissection of Plant Immune Signaling Pathways -- Identification of Phosphorylation and Protein Interactions of Plant Pattern Recognition Receptors Through Immunoprecipitation -- Plant-Based Ligand-Depletion Binding Assay for Assessing Pattern Recognition Receptor-Ligand Interactions -- Plate Reader-Based Quantification of Cellular Calcium Dynamics in Immune Responses Using the Genetically-Encoded Calcium Indicator GCaMP -- Visualization of Calcium Decoding via a FRET-Based Reporter of Calcium-Decoder Conformation -- Visualization and Quantification of Apoplastic ROS by Plant Immune NADPH Oxidase Utilizing Chemiluminescence -- Detection of Phosphorylated NADPH Oxidase by Antibody During Plant Immune Responses -- The Application of RenSeq for Pan-NLRome Analysis -- Blue Native-PAGE Assay of NLR Protein Complexes Combined with Genetic Complementation in Nicotiana benthamiana -- Quantification of NLR-Induced Cell Death Using Rice Protoplasts -- Split-TurboID-Based Proximity Labeling for Probing the Composition of Spatiotemporally Defined Protein Complexes In Planta -- Virus-Induced Gene Silencing for Identifying Autoimmune Regulators in Arabidopsis -- Bioinformatics Workflow for Co-Transcriptome Analysis of Plant-Bacterial Interactions -- Nuclei Isolation from Mature Leaves of Arabidopsis thaliana Infected by Bacterial Pathogens for Single-Nucleus Multiomics -- Multimodal Analysis of Phytoalexin Synthesis in Arabidopsis by Mass Spectrometry Imaging and Fluorescent Microscopy -- Bioluminescence-Based Growth Quantification of the Phytopathogenic Bacterium Pseudomonas syringae pv. tomato DC3000 -- Genome Editing for Developing Disease-Resistant Plants.
This volume explores plant immunity through protocols for recently established genetic and biochemical techniques to elucidate immune mechanisms mediated by pattern-recognition receptors (PRRs) and nucleotide-binding leucine-rich repeat receptors (NLRs). The book covers the identification of PRRs, their associated downstream immune regulators, PRR-ligand interaction-based immune responses, identification of NLR complex components, pan-NLRome analysis, resistosome assays, and cell death evaluation. Additionally, it presents protocols for co-transcriptome analysis of plant-pathogen interactions, single-nucleus multi-omics, metabolite imaging, and bioluminescence-based quantification of pathogen growth, as well as genome editing tools that contribute to the development of disease-resistant plants. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Immunity: Methods and Protocols serves as an ideal guide for researchers aiming to accelerate ongoing research and advances in the field of plant immunity.
9781071651384
10.1007/978-1-0716-5138-4 doi
Plants--Disease and pest resistance.
Botany.
Plant Immunity.
Plant Science.
SB750 QR185.2-.6
571.9642